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Peptide mapping protocols that detail the entire protein (100% sequence coverage) are required to prove molecular structure as well as determine post-translational modifications (PTMs). Complex protein digests require high peak capacity and high-resolution separations. Our peptide mapping workflow solution includes a fast, reproducible protein digestion protocol and powerful yet simple peptide mapping software, combined with the Thermo Scientific Vanquish Flex or Thermo Scientific Vanquish Horizon UHPLC system.
Peptide mapping is a routine method to characterize biopharmaceutical proteins that have been enzymatically digested resulting in complex samples. Peptide mapping methods are often developed and evaluated with combined UV and MS detection to identify, characterize, confirm and quantify the peptides. Coupling the Vanquish UHPLC with a Thermo Scientific Orbitrap mass spectrometer and Thermo Scientific BioPharma Finder software provides accurate, in-depth characterization and relative quantification of biotherapeutic peptides for streamline peptide mapping confidence. Further improve your productivity by implementing tandem LC workflows with the Vanquish Duo UHPLC using existing validated methods while reducing carryover, with extended column washes, without sacrificing throughput.
Type | Title |
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Analyte Guru | A Brief History of and Guide to Peptide Mapping |
Analyte Guru | Ahoy! Do I Need Satellite Navigation for my Peptide Map? |
Analyte Guru | Not All Who Wander Are Lost |
Analyte Guru | A Brief History of and Guide to Peptide Mapping |
AppsLab | AppsLab Library - Peptide Mapping |
Application Note | LC-UV-MS Peptide Mapping Development for Easy Transfer to LC-UV QA/QC |
Application Note | High throughput Peptide Mapping by UHPLC-MS |
Article | Developments in Peptide Mapping Technology for the Biopharmaceutical Industry (The Analytical Scientist) |
Article | 'Ultra' Biotherapeutic Characterisation |
Article | High-Throughput, High-Resolution Peptide Maps |
Poster Note | New Integrated Informatics Solution for Protein Biotherapeutics Characterization |
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Technical Note | Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses |