mAb characterization, aggregate, fragment and titer analyses

Characterization of mAbs and their aggregates, fragments, and titers

Extensive development of protein and monoclonal antibody (mAb) therapeutics currently underway requires innovative and efficient tools that characterize and quantify structural variants and modifications, monitor product stability and demonstrate safety and efficacy. These specifically designed Thermo Scientific MAbPac columns provide a full range of high resolution, high efficiency mAb analyses by LC and LC-MS.

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Monoclonal antibody LC columns
Reversed phase monoclonal antibody columns
Reversed phase monoclonal antibody columns

Now you can use reversed-phase conditions for higher resolution separation of intact mAbs and fragments by HPLC and LC-MS.

Hydrophobic interaction chromatography (HIC) mAb columns

Use these columns for mAb, mAb fragment, antibody-drug aggregate, and related biologic separations.

Hydrophobic interaction chromatography (HIC) mAb columns
Affinity monoclonal antibody columns
Affinity monoclonal antibody columns

Use these columns for fast and accurate titer analysis of monoclonal antibodies in harvest cell cultures.

Strong cation exchange monoclonal antibody columns

Use these columns for high-resolution, high-efficiency analysis of mAbs and associated variants.

Strong cation exchange monoclonal antibody columns
Size exclusion mAb columns
Size exclusion monoclonal antibody columns

Choose these columns for optimal mAb analysis, including the high-resolution separation of monomers, aggregates and fragments.

Which column to choose for monoclonal antibody analysis?

Analysis

Description

Columns & Buffers

mAb Capture & titer analysis
  • mAb titer determination (concentration) & screening
  • mAb capture for analysis workflows
Aggregate analysisRouting screening for mAb aggregates and fragments
Charge variant analysisRoutine charge variant profiling/screening, including lysine truncation, deamidation & acylation
Methionine & tryptophan oxidationTargeted analysis of methionine and tryptophan oxidation
Antibody drug conjugate (ADC) analysisADC DAR analysis
Intact mAb & fragment analysis
  • Light chain (LC) and heavy chain (HC) analysis
  • Fab and Fc analysis
  • scFc and F(ab’)2 analysis
Sequence & structural analysis
  • Primary sequence analysis
  • peptide mapping
  • peptide & glycopeptides structural & linkage analysis
Glycan profilingProfiling of released glycans
Chromatography Consumables Resources and Information

Find interactive tools and calculators, guides to select the right products, equivalent product finders, and get in contact with our consumables technical support team to answer your application questions.

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Workflow product selector

Select Chromatography Consumables and Columns for your Pharma, Biopharma, Clinical, Food & Beverage or Environmental workflow.

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Featured monoclonal antibody analysis videos

This interactive tool will guide you through the products we offer for antibody analysis and purification. Start by selecting an area of interest to display relevant information. Continue making selections that interest you.
 

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On-demand Consumables for chromatography webinar series: Learn at your own pace on a wide range of topics from column troubleshooting, method transfer, to tips for successful low-flow and more​

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Resources
CMD SchemaApp code