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AppsLab Library of Analytical Applications
Liquid chromatography learning center
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System resources
Vanquish Horizon UHPLC System
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Horizon UHPLC System
Vanquish Duo HPLC and UHPLC systems
Application notes
Quantitation of acetylcholinesterase inhibitors
Metoprolol and Select Impurities Analysis Using a Hydrophilic Interaction Chromatography Method with Combined UV and Charged Aerosol Detection
Increased Long-term Stability of Peptide Mapping Using the Vanquish UHPLC System
UHPLC Method Development for Simultaneous Determination of Antihypertensive Combination Agents
Transfer of an EP method for mebendazole from a Waters Acquity UPLC system to a Vanquish Horizon UHPLC system
Simultaneous quantification of nevirapine and low-level impurities (DAD FG)
Impurity analysis of L-aspartic acid and glycine by HPLC-UV-CAD
Fast and Ultrafast LC-MS/MS Methods for Robust and Reliable Analysis of Pesticides in Food Using the Vanquish UHPLC System
Providing the Highest Retention Time and Peak Area Reproducibility for Maximal Confidence in Peptide Mapping Experiments
High-Throughput Peptide Mapping with the Vanquish UHPLC System and the Q Exactive HF Mass Spectrometer
Case studies
Simplifying complexity in therapeutic protein drug development
High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow
Deploying the multi-attribute method (MAM) across sites at Pfizer
Peptide mapping of challenging monoclonal antibodies
Fighting Food Fraud - a unique approach to tackle extra virgin olive oil adulteration
Empowering success by adopting new LC instruments within an existing network
Increasing throughput of native SEC-MS in biopharmaceutical development using a tandem UHPLC setup
Compendiums
Biopharmaceutical Characterisation Compendium
Productivity enhancement with liquid chromatography solutions
Flyers and infographics
LC System Portfolio
Proactive sample handling solutions
LC Portfolio for LC-MS Applications
Eight reasons to consider
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Product spotlight
How solvent delivery technology can improve confidence in peak identification and quantification
Best in UHPLC Column Thermostatting to Fit All Needs
Ensuring robust sequences through automatic continuous management of HPLC mobile phases and waste
Technical notes
Intelligent UHPLC pump stroke control
A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures
How to Realize LC-MS Quantitation with Chromeleon 7.2 CDS
Boosting Trace Detection Performance with the Vanquish Diode Array Detector and High-Sensitivity LightPipe Flow Cell
Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses
Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity
Development of Ultra-fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants
How to Realize LC-MS Quantitation with Chromeleon 7.2 CDS
Selective analysis of co-eluting isobaric compounds with single quadrupole mass detection
Videos
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Webinars
Liquid chromatography tips and tricks
Vanquish Flex UHPLC Systems
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Flex UHPLC Systems
Vanquish Duo HPLC and UHPLC systems
Application notes
SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals
A universal chromatography method for aggregate analysis of monoclonal antibodies
The importance of correct UHPLC instrument setup for protein aggregate analysis by size-exclusion chromatography
HRAM LC-MS method for the determination of nitrosamine impurities in drugs
Analysis of underivatized amino acids in wines by solid phase extraction and HILIC-MS
Determination of underivatized aflatoxins B2, B1, G2, and G1 in ground hazelnuts by immunoaffinity solid-phase extraction with HPLC-FLD detection
Determination of olive oil purity based on triacylglycerols profiling by UHPLC-CAD and principal component analysis
Amino acid analysis of mammalian cell culture medium by liquid chromatography with UV and fluorescence detection and derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
Accurate and Reliable Quantitation of p-Toluenesulfonates Genotoxic Impurities in an Active Pharmaceutical Ingredient by HPLC-UV
Identity confirmation and accurate quantitation of a genotoxic impurity in an active pharmaceutical ingredient by UHPLC-UV coupled to a single quadrupole mass detector
Validation of routine polycyclic aromatic hydrocarbons analysis in waters
Case studies
Simple, Robust, High Quality Intact Mass Analysis—A Biosimilars Case Study
Characterizing Therapeutic Proteins for Drug Discovery and Development
Leaders in characterization: Bio-Techne Corporation
Fighting food fraud -- a unique approach to tackle extra virgin olive oil adulteration
Empowering success by adopting new LC instruments within an existing network
Characterizing Therapeutic Proteins for Drug Discovery and Development
Bringing Biosimilar Therapeutics To Market Faster – A CRO Case Study
Using simplified walk-up software to bring UHPLC-MS to the organic synthesis lab
Leaders in characterization
Compendiums
Biopharmaceutical Characterisation Compendium
Productivity enhancement with liquid chromatography solutions
Flyers and infographics
LC System Portfolio
Proactive sample handling solutions
LC Portfolio for LC-MS Applications
Eight reasons to consider
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Product spotlight
Outstanding Long-term Reproducibility for Utmost Data Confidence
Technical notes
A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures
Transferring a Method for the Characterization of PTMs from the UltiMate 3000 BioRS System to the Vanquish Flex UHPLC System
Selective analysis of co-eluting isobaric compounds with single quadrupole mass detection
Videos
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Webinars
Liquid chromatography tips and tricks
Vanquish Core HPLC Systems
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Core HPLC System
Application notes
Transfer of an EP Method for Chlorhexidine Impurity Analysis from a Shimadzu Nexera-i system
Straightforward transfer of an EP method for impurity analysis of chlorhexidine from an Agilent 1260 Infinity LC system to a Vanquish Core HPLC system
Transfer of a compendial LC method for impurity analysis of chlorhexidine from a Waters Alliance HPLC system to a Vanquish Core HPLC system
Seamless transfer of a compendial LC method for impurity analysis of chlorhexidine from an UltiMate 3000 Standard HPLC system to a Vanquish Core HPLC system
Simultaneous determination of vitamin A, D and four vitamin E isomers in infant formula by offline solid phase extraction and online two-dimensional liquid chromatography
Improving peak results using a custom injection program to reduce solvent strength prior to sample injection
Forced-degradation evaluation of erythromycin by HPLC and single quadrupole mass spectrometry
Method validation based on ICH guidelines of a USP assay method of acetaminophen
Analysis of cefprozil and related impurities by reversed-phase liquid chromatography with UV detection
Straightforward implementation of a compendial LC method for metolazone impurity analysis with the Vanquish Core HPLC system
Compendiums
Productivity enhancement with liquid chromatography solutions
Flyers and infographics
LC System Portfolio
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Product spotlight
System Health Checks
Automatic continuous management of HPLC mobile phases and waste
Technical notes
Global round robin test of thiopental EP method performance on identical HPLC systems
Long-term robustness of thiopental Ph. Eur. method performance on modern HPLC instrumentation
Physical adjustment of gradient delay volume as a tool for successful transfer of HPLC methods
Videos
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Webinars
Building a more Efficient Routine Lab: Approaches to Modernizing HPLC Methods with New Technologies
Liquid chromatography tips and tricks
White papers
Thermo Scientific Vanquish Core HPLC System Cost of Ownership
Vanquish Duo UHPLC Systems for Dual LC
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Duo HPLC and UHPLC Systems
Application notes
Doubling the throughput of long chromatographic methods by using a novel dual LC workflow
High-throughput protein aggregate analysis of monoclonal antibiodies using a novel dual-channel UHPLC instrument
Simultaneous analysis of monoclonal antibodies using a novel dual-channel UHPLC instrument and orthogonal chromatography
Simultaneous determination of water and fat-soluble vitamins in tablets and energy drinks by using a novel Vanquish Flex Duo system for Dual LC
Simultaneous high-performance and ultra-high-performance liquid chromatographic analysis of acetominophen impurities using a single instrument
Case studies
Using simplified walk-up software to bring UHPLC-MS to the organic synthesis lab
Empowering success by adopting new LC instruments within an existing network
Compendiums
Biopharmaceutical Characterisation Compendium
Productivity enhancement with liquid chromatography solutions
Flyers and infographics
LC System Portfolio
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems
Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity
Videos
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Liquid chromatography tips and tricks
Liquid chromatography tips and tricks
Webinars
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Vanquish Duo HPLC and UHPLC Systems for Tandem LC or LC-MS
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Duo HPLC and UHPLC Systems
Application notes
Increasing the throughput for native SEC-MS in biopharmaceutical development using a Vanuish Duo Tandem LC setup
High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow
Compendiums
Productivity enhancement with liquid chromatography solutions
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses
Videos
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Webinars
Liquid chromatography tips and tricks
Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Duo HPLC and UHPLC Systems
Discover what you’re missing - Thermo Scientific Charged Aerosol Detectors
Application notes
Quantification of paclitaxel, its degradants, and related substances using UHPLC with charged aerosol detection
Compendiums
Productivity enhancement with liquid chromatography solutions
Flyers and infographics
List of Compendial Methods for Charged Aerosol Detectors
Online tools
HPLC and UHPLC method development calculator
Product spotlight
Achieving standard-free quantitation
Technical notes
Why use charged aerosol detection with inverse gradient?
Videos
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Webinars
Evaluating HPLC method transfer with charged aerosol detection and inverse gradient
Liquid chromatography tips and tricks
Vanquish Duo UHPLC Systems for Dual LC-MS
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Duo UHPLC Systems
Application notes
Increasing throughput of native SEC-MS in biopharmaceutical development using a tandem UHPLC setup
High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow
Case studies
Deploying the multi-attribute method (MAM) across sites at Pfizer
Compendiums
Productivity enhancement with liquid chromatography solutions
Flyers and infographics
LC System Portfolio
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Product spotlight
Thermo Scientific Vanquish Duo system for Transcend Duo LX-2
Technical notes
Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses
Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems
Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity
Videos
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Webinars
Understand Product Quality Attributes of Biotherapeutics through a Multi-Attribute Method (MAM) Lab-of-the-Future
HR Multi-Attribute Method for Biopharma Analysis
Selecting the Right Solvents for Your Separations
Implementation of Dual Channel Chromatography to Improve Productivity in the Analysis of Pesticide Residues in Food
Vanquish Online SPE HPLC and UHPLC Systems
Brochures
Vanquish HPLC and UHPLC Systems
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures
Quantification of free metanephrines in human plasma by LC-MS/MS for clinical research
Fast and accurate quantitation of intact IGF-1 from serum by HRAM LC-MS for clinical research
Videos
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Vanquish Loop Heart-Cut 2D-LC Systems
Brochures
Vanquish HPLC and UHPLC Systems
Application notes
Simultaneous determination of vitamin A, D and four vitamin E isomers in infant formula by offline solid phase extraction and online two-dimensional liquid chromatography
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity
Videos
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Vanquish Trap Heart-Cut 2D-LC Systems
Brochures
Vanquish HPLC and UHPLC Systems
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems
Videos
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Vanquish Simple Switch 2D-LC Systems
Brochures
Vanquish HPLC and UHPLC Systems
Vanquish Duo HPLC and UHPLC Systems
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems
Videos
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UltiMate 3000 RS UHPLC Systems
Brochures
UltiMate 3000 RS UHPLC System
UltiMate 3000 BioRS UHPLC System
Application notes
Analysis of Non-Steroidal Anti-Inflammatory Drugs Using a Highly Pure, High Surface Area C18 HPLC Column
Automated method development in HPLC for the quantitative determination of catechins in tea
Separation of isomers of vitamin K1 using normal phase HPLC
Compendiums
Productivity enhancement with liquid chromatography solutions
Posters
Evaluation of Variable Data Independent Acquisition (vDIA) Approach for Nontarget Screening of Veterinary Drugs in Animal Feed
Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control
A Novel Strategy for the Discovery and Characterization of New Natural Products from Traditional Chinese Medicine by Using Benchtop Orbitrap Mass Spectrometer
Product spotlight
Liquid chromatography systems -- the latest LightPipe technology for the UltiMate 3000 Platform
Technical notes
A universal tool for method transfer from HPLC to UHPLC
White papers
How does the analysis of biomolecules benefit from UHPLC?
UltiMate 3000 SD HPLC Systems
Brochures
UltiMate 3000 SD HPLC System
Application notes
Seamless transfer of a compendial LC method for impurity analysis of chlorhexidine from an UltiMate 3000 Standard HPLC system to a Vanquish Core HPLC system
Online tools
HPLC and UHPLC method development calculator
HPLC Total Cost of Ownership Calculator
Technical notes
Transfer of a Heart Disease Treatment Analysis from an Agilent 1100 System to an UltiMate 3000 HPLC System
White papers
An instrument parameter guide for successful (U)HPLC method transfer
UltiMate 3000 Basic HPLC Systems
Brochures
UltiMate 3000 Basic Automated HPLC Systems
UltiMate 3000 Semi-Preparative HPLC Systems
Online tools
Preparative HPLC column method Transfer Tool
EASY-nLC 1200 System
Brochures
EASY-nLC 1200 System
Specification sheets
EASY-nLC 1200 System
Guide to configuring your Thermo Scientific nano LC for mass spectrometric analysis
Application notes
EASY-Spray best practices
EASY-Spray troubleshooting
Establishing a stable spray on EASY-Spray columns
Improving Retention Time Precision and Chromatography of Early Eluting Peptides with Acetonitrile/Water Blends as Solvent B
Pushing the Limits of Bottom-Up Proteomics with State-Of-The-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes
A novel 75 cm column size gives increased resolution and better sequence coverage
Flyers and infographics
LC System Portfolio
LC Portfolio for LC-MS Applications
Technical notes
EASY-nLC 1200 System – Peak nano LC performance at your fingertips
UltiMate 3000 RSLCnano System
Brochures
UltiMate 3000 RSLCnano System
Specification sheets
UltiMate 3000 RSLCnano System
Guide to configuring your Thermo Scientific nano LC for mass spectrometric analysis
Application notes
A Highly Sensitive and Robust 150 μm Column to Enable High-Throughput Proteomics
LC/MS Analysis of the Monoclonal Antibody Rituximab Using the Q Exactive Benchtop Orbitrap Mass Spectrometer
Use of a MAbPac RP Capillary Column to Monitor Glycosylation Site Occupancy in Therapeutics
Flyers and infographics
LC System Portfolio
LC Portfolio for LC-MS Applications
UltiMate 3000 RSLCnano System with ProFlow Technology
Thermo Scientific UltiMate 3000 RSLCnano system with ProFlow technology
Technical notes
Capillary-Flow LC-MS: Combining High Sensitivity, Robustness, and Throughput
Capillary-Flow LC-UV Sample Quality Control for Low-Flow LC-MS Based Proteomics
High-Throughput Capillary-Flow LC-MS Proteomics with Maximum MS Utilization
High-Throughput Tandem Capillary-Flow LC-MS for Maximum MS Utilization
Maximize Productivity in Proteome Profiling: Combining Intelligent Tandem nanoLC-MS Methods and a Novel Double-Barrel ESI Source for 100% MS Utilization
Online 2D-nanoLC Hyphenated with High-Resolution Accurate-mass Orbitrap Mass Spectrometry for Comprehensive and Robust Proteome Profiling
Tailored High-Throughput Low-Flow LC-MS Methods for Large Sample Cohort Analysis
Tandem nanoLC-MS: maximum MS utilization for deep-dive proteomic analysis
Transcend LX Multichannel UHPLC Systems
Compendiums
Maximize throughput with multichannel liquid chromatography
Flyers and infographics
LC System Portfolio
Guides
Transcend Multichannel LC maintenance guide
Aria MX User Guide
Webinars
Maximize LC-MS/MS throughput, economy and efficiency using multichannel & on-line sample cleanup technologies
Transcend TLX TurboFlow UHPLC Systems
Compendiums
Maximize throughput with multichannel liquid chromatography
Flyers and infographics
LC System Portfolio
Guides
Transcend Multichannel LC
Aria MX User Guide
Webinars
Maximize LC-MS/MS throughput, economy and efficiency using multichannel & on-line sample cleanup technologies
Component resources
Pumps
Specification sheets
UltiMate 3000 Solvent Racks
UltiMate 3000 Pumps
Vanquish Pumps
UltiMate 3000 RSLCnano UHPLC System
Flyer
UltiMate 3000 RSLCnano System with ProFlow Technology
Technical note
Intelligent UHPLC pump stroke control
Trifluoroacetic acid performance of the Vanquish Flex Binary UHPLC system
Tune your mixing volume for gradient generation to achieve better detection limits
Video
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Autosamplers
Specification sheets
UltiMate 3000 Well Plate Autosampler
Vanquish Split Sampler
Vanquish Charger
ACC-3000 Autosampler Column Compartment
Vanquish Sample Container Kit
Application note
Lifetime stability of size exclusion chromatography columns for protein aggregate analysis
Column Compartments
Specification sheets
Vanquish Column Compartments
UltiMate 3000 Thermostatted Column Compartment
ACC-3000 Autosampler Column Compartment
UltiMate 3000 RSLCnano UHPLC System
White paper
The Role of Temperature and Column Thermostatting in Liquid Chromatography
Detectors
Charged Aerosol Detector (CAD)
Brochures
Charged Aerosol Detectors
Specification sheets
Vanquish Charged Aerosol Detectors
Corona Veo Charged Aerosol Detector
Peak Scientific Nitrogen Gas Generation System
Application notes
Customer application Note: Impurity analysis of L-aspartic acid and glycine by HPLC-UV-CAD
Comprehensive analysis of components and degradation products in coolants
Reliable verification and quantification of the presence of extraneous compounds present in a sample, such as impurities, degradation products, or extractables and leachables
Compendiums
HPLC-CAD Excipients applications notebook. Complex substances, universal chromatographic analysis
CAD Bibliography
HPLC-CAD Surfactants and Emulsifiers Applications Notebook
Technical notes
Guidelines for method transfer and optimization—from earlier model Corona detectors to Corona Veo and Vanquish charged aerosol detectors
Charged aerosol detection - factors affecting uniform analyte response
Why use charged aerosol detection with inverse gradient?
Charged aerosol detection – use of the power function and robust calibration practices to achieve the best quantitative
Video
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Webinars
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Evaluating HPLC method transfer with charged aerosol detection and inverse gradient
Diode Array Detector (DAD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD)
Specification sheets
Vanquish Diode Array Detectors and Multiple Wavelength Detectors
Vanquish Variable Wavelength Detectors
UltiMate 3000 Variable Wavelength Detectors
UltiMate 3000 Diode Array and Multiple Wavelength Detectors
Application notes
Use of a MAbPac RP capillary column to monitor glycosylation site occupancy in therapeutics
High Salt Gradient Analysis of Post-Translational Modifications - Deamidation Monitoring
Accurate and reliable quantitation of p-toluenesulfonates genotoxic impurities in an active pharmaceutical ingredient by HPLC-UV
Fast Nevirapine Impurity Profiling Using UHPLC-DAD
Simultaneous quantification of nevirapine and low-level impurities
Increased Long-term Stability of Peptide Mapping Using the Vanquish UHPLC System
Product spotlight
Liquid chromatography systems -- the latest LightPipe technology for the UltiMate 3000 Platform
Technical notes
Multiple Wavelength Data Acquisition with the Vanquish Variable Wavelength Detector
Boosting Trace Detection Performance with the Vanquish Diode Array Detector and High-Sensitivity LightPipe Flow Cell
Fluorescence Detector (FLD)
Specification sheets
Vanquish Fluorescence Detectors
UltiMate 3000 Fluoresecence Detectors
Application notes
Utilizing the Native Fluorescence of Monoclonal Antibodies for the Sensitive Detection of Charge Variants
Customer Application Note: Validation of routine polycyclic aromatic hydrocarbons analysis in waters
Technical notes
Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors
Fluorescence Method Development Handbook
Combining fluorescence detection with UHPLC: an overview of the technical requirements
Video
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Mass Spectrometer (MS), Single Quadrupole
Brochure
ISQ Brochure
Specification sheets
ISQ-EC Single Quadrupole Mass Spectrometer
ISQ-EM Single Quadrupole Mass Spectrometer
Application notes
Analysis of underivatized amino acids in wines by solid phase extraction and HILIC-MS
A multi-detector platform comprising UV/Vis, charged aerosol, and single quadrupole mass spectrometric detection for comprehensive sample analysis
Detection of oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) in APCI mode with a single quadrupole mass spectrometer
Impurity profiling of the synthetic peptide LL-37 using high-performance liquid chromatography with combined UV and single quadrupole mass spectrometric detection
Oligonucleotide characterization for quality control and increased productivity by single quadrupole mass spectrometer with extended mass range
Identity confirmation and accurate quantitation of a genotoxic impurity in an active pharmaceutical ingredient by UHPLC-UV coupled to a single quadrupole mass detector
Accelerate method scouting with the Thermo Scientifc ISQ EC single quadrupole mass spectrometer and the Thermo Scientific Vanquish Flex Quaternary UHPLC
Achieve confident impurity detection with the Thermo Scientifc ISQ EC single quadrupole mass spectrometer
Achieve confident sysnthesis control with the Thermo Scientifc ISQ EC single quadrupole mass spectrometer
Technical notes
The benefits of adding single quadrupole mass detection to liquid chromatography
Quality control of oligonucleotides with a single quadrupole mass spectrometer
Quick and easy tuning and troubleshooting of ISQ EC and ISQ EM mass spectrometers
Selective analysis of co-eluting isobaric compounds with single quadrupole mass detection
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