HPLC and UHPLC Resources

Excellence built into each innovative liquid chromatography system.

Tools, methods, applications and learning resources

AppsLab Library of Analytical Applications

Liquid chromatography learning center

Vanquish HPLC and UHPLC resource library

HPLC Manuals and Quick Installation Guides

System resources

Vanquish Horizon UHPLC System

Vanquish HPLC and UHPLC Systems

Vanquish Horizon UHPLC System

Vanquish Duo HPLC and UHPLC systems

Quantitation of acetylcholinesterase inhibitors

Metoprolol and Select Impurities Analysis Using a Hydrophilic Interaction Chromatography Method with Combined UV and Charged Aerosol Detection

Increased Long-term Stability of Peptide Mapping Using the Vanquish UHPLC System

UHPLC Method Development for Simultaneous Determination of Antihypertensive Combination Agents

Transfer of an EP method for mebendazole from a Waters Acquity UPLC system to a Vanquish Horizon UHPLC system

Simultaneous quantification of nevirapine and low-level impurities (DAD FG)

Impurity analysis of L-aspartic acid and glycine by HPLC-UV-CAD

Fast and Ultrafast LC-MS/MS Methods for Robust and Reliable Analysis of Pesticides in Food Using the Vanquish UHPLC System

Providing the Highest Retention Time and Peak Area Reproducibility for Maximal Confidence in Peptide Mapping Experiments

High-Throughput Peptide Mapping with the Vanquish UHPLC System and the Q Exactive HF Mass Spectrometer

Simplifying complexity in therapeutic protein drug development

High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow

Deploying the multi-attribute method (MAM) across sites at Pfizer

Peptide mapping of challenging monoclonal antibodies

Fighting Food Fraud - a unique approach to tackle extra virgin olive oil adulteration

Empowering success by adopting new LC instruments within an existing network

Increasing throughput of native SEC-MS in biopharmaceutical development using a tandem UHPLC setup

Biopharmaceutical Characterisation Compendium

Productivity enhancement with liquid chromatography solutions

LC System Portfolio

Proactive sample handling solutions

LC Portfolio for LC-MS Applications

Eight reasons to consider

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

How solvent delivery technology can improve confidence in peak identification and quantification

Best in UHPLC Column Thermostatting to Fit All Needs

Ensuring robust sequences through automatic continuous management of HPLC mobile phases and waste

Intelligent UHPLC pump stroke control

A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

How to Realize LC-MS Quantitation with Chromeleon 7.2 CDS

Boosting Trace Detection Performance with the Vanquish Diode Array Detector and High-Sensitivity LightPipe Flow Cell

Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses

Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity

Development of Ultra-fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants

How to Realize LC-MS Quantitation with Chromeleon 7.2 CDS

Selective analysis of co-eluting isobaric compounds with single quadrupole mass detection

Liquid chromatography tips and tricks

Vanquish Flex UHPLC Systems

Vanquish HPLC and UHPLC Systems

Vanquish Flex UHPLC Systems

Vanquish Duo HPLC and UHPLC systems

SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals

A universal chromatography method for aggregate analysis of monoclonal antibodies

The importance of correct UHPLC instrument setup for protein aggregate analysis by size-exclusion chromatography

HRAM LC-MS method for the determination of nitrosamine impurities in drugs

Analysis of underivatized amino acids in wines by solid phase extraction and HILIC-MS

Determination of underivatized aflatoxins B2, B1, G2, and G1 in ground hazelnuts by immunoaffinity solid-phase extraction with HPLC-FLD detection

Determination of olive oil purity based on triacylglycerols profiling by UHPLC-CAD and principal component analysis

Amino acid analysis of mammalian cell culture medium by liquid chromatography with UV and fluorescence detection and derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate

Accurate and Reliable Quantitation of p-Toluenesulfonates Genotoxic Impurities in an Active Pharmaceutical Ingredient by HPLC-UV

Identity confirmation and accurate quantitation of a genotoxic impurity in an active pharmaceutical ingredient by UHPLC-UV coupled to a single quadrupole mass detector

Validation of routine polycyclic aromatic hydrocarbons analysis in waters

Simple, Robust, High Quality Intact Mass Analysis—A Biosimilars Case Study

Characterizing Therapeutic Proteins for Drug Discovery and Development

Leaders in characterization: Bio-Techne Corporation

Fighting food fraud -- a unique approach to tackle extra virgin olive oil adulteration

Empowering success by adopting new LC instruments within an existing network

Characterizing Therapeutic Proteins for Drug Discovery and Development

Bringing Biosimilar Therapeutics To Market Faster – A CRO Case Study

Using simplified walk-up software to bring UHPLC-MS to the organic synthesis lab

Leaders in characterization

Biopharmaceutical Characterisation Compendium

Productivity enhancement with liquid chromatography solutions

LC System Portfolio

Proactive sample handling solutions

LC Portfolio for LC-MS Applications

Eight reasons to consider

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Outstanding Long-term Reproducibility for Utmost Data Confidence

A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

Transferring a Method for the Characterization of PTMs from the UltiMate 3000 BioRS System to the Vanquish Flex UHPLC System

Selective analysis of co-eluting isobaric compounds with single quadrupole mass detection

Liquid chromatography tips and tricks

Vanquish Core HPLC Systems

Vanquish HPLC and UHPLC Systems

Vanquish Core HPLC System

Transfer of an EP Method for Chlorhexidine Impurity Analysis from a Shimadzu Nexera-i system

Straightforward transfer of an EP method for impurity analysis of chlorhexidine from an Agilent 1260 Infinity LC system to a Vanquish Core HPLC system

Transfer of a compendial LC method for impurity analysis of chlorhexidine from a Waters Alliance HPLC system to a Vanquish Core HPLC system

Seamless transfer of a compendial LC method for impurity analysis of chlorhexidine from an UltiMate 3000 Standard HPLC system to a Vanquish Core HPLC system

Simultaneous determination of vitamin A, D and four vitamin E isomers in infant formula by offline solid phase extraction and online two-dimensional liquid chromatography

Improving peak results using a custom injection program to reduce solvent strength prior to sample injection

Forced-degradation evaluation of erythromycin by HPLC and single quadrupole mass spectrometry

Method validation based on ICH guidelines of a USP assay method of acetaminophen

Analysis of cefprozil and related impurities by reversed-phase liquid chromatography with UV detection

Straightforward implementation of a compendial LC method for metolazone impurity analysis with the Vanquish Core HPLC system

Productivity enhancement with liquid chromatography solutions

LC System Portfolio

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

System Health Checks

Automatic continuous management of HPLC mobile phases and waste

Global round robin test of thiopental EP method performance on identical HPLC systems

Long-term robustness of thiopental Ph. Eur. method performance on modern HPLC instrumentation

Physical adjustment of gradient delay volume as a tool for successful transfer of HPLC methods

Building a more Efficient Routine Lab: Approaches to Modernizing HPLC Methods with New Technologies

Liquid chromatography tips and tricks

Thermo Scientific Vanquish Core HPLC System Cost of Ownership

Vanquish Duo UHPLC Systems for Dual LC

Vanquish HPLC and UHPLC Systems

Vanquish Duo HPLC and UHPLC Systems

Doubling the throughput of long chromatographic methods by using a novel dual LC workflow

High-throughput protein aggregate analysis of monoclonal antibiodies using a novel dual-channel UHPLC instrument

Simultaneous analysis of monoclonal antibodies using a novel dual-channel UHPLC instrument and orthogonal chromatography

Simultaneous determination of water and fat-soluble vitamins in tablets and energy drinks by using a novel Vanquish Flex Duo system for Dual LC

Simultaneous high-performance and ultra-high-performance liquid chromatographic analysis of acetominophen impurities using a single instrument

Using simplified walk-up software to bring UHPLC-MS to the organic synthesis lab

Empowering success by adopting new LC instruments within an existing network

Biopharmaceutical Characterisation Compendium

Productivity enhancement with liquid chromatography solutions

LC System Portfolio

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems

Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity

Liquid chromatography tips and tricks

Vanquish Duo HPLC and UHPLC Systems for Tandem LC or LC-MS

Vanquish HPLC and UHPLC Systems

Vanquish Duo HPLC and UHPLC Systems

Increasing the throughput for native SEC-MS in biopharmaceutical development using a Vanuish Duo Tandem LC setup

High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow

Productivity enhancement with liquid chromatography solutions

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses

Liquid chromatography tips and tricks

Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient

Vanquish HPLC and UHPLC Systems

Vanquish Duo HPLC and UHPLC Systems

Discover what you’re missing - Thermo Scientific Charged Aerosol Detectors

Quantification of paclitaxel, its degradants, and related substances using UHPLC with charged aerosol detection

Productivity enhancement with liquid chromatography solutions

List of Compendial Methods for Charged Aerosol Detectors

HPLC and UHPLC method development calculator

Achieving standard-free quantitation

Why use charged aerosol detection with inverse gradient?

Evaluating HPLC method transfer with charged aerosol detection and inverse gradient

Liquid chromatography tips and tricks

Vanquish Duo UHPLC Systems for Dual LC-MS

Vanquish HPLC and UHPLC Systems

Vanquish Duo UHPLC Systems

Increasing throughput of native SEC-MS in biopharmaceutical development using a tandem UHPLC setup

High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow

Deploying the multi-attribute method (MAM) across sites at Pfizer

Productivity enhancement with liquid chromatography solutions

LC System Portfolio

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Thermo Scientific Vanquish Duo system for Transcend Duo LX-2

Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses

Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems

Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity

Understand Product Quality Attributes of Biotherapeutics through a Multi-Attribute Method (MAM) Lab-of-the-Future

HR Multi-Attribute Method for Biopharma Analysis

Selecting the Right Solvents for Your Separations

Implementation of Dual Channel Chromatography to Improve Productivity in the Analysis of Pesticide Residues in Food

Vanquish Online SPE HPLC and UHPLC Systems

Vanquish HPLC and UHPLC Systems

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

A pre-concentration and online solid phase extraction setup for the LC-MS analysis of therapeutic protein mixtures

Quantification of free metanephrines in human plasma by LC-MS/MS for clinical research

Fast and accurate quantitation of intact IGF-1 from serum by HRAM LC-MS for clinical research

Vanquish Loop Heart-Cut 2D-LC Systems

Vanquish HPLC and UHPLC Systems

Simultaneous determination of vitamin A, D and four vitamin E isomers in infant formula by offline solid phase extraction and online two-dimensional liquid chromatography

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Complementary Dual LC as a convenient alternative to multiple heart-cut 2D-LC for samples of medium complexity

Vanquish Trap Heart-Cut 2D-LC Systems

Vanquish HPLC and UHPLC Systems

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems

Vanquish Simple Switch 2D-LC Systems

Vanquish HPLC and UHPLC Systems

Vanquish Duo HPLC and UHPLC Systems

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Flexible HPLC instrument setups for double usage as one heart-cut-2D-LC system or two independent 1D-LC systems

UltiMate 3000 RS UHPLC Systems

UltiMate 3000 RS UHPLC System

UltiMate 3000 BioRS UHPLC System

Analysis of Non-Steroidal Anti-Inflammatory Drugs Using a Highly Pure, High Surface Area C18 HPLC Column

Automated method development in HPLC for the quantitative determination of catechins in tea

Separation of isomers of vitamin K1 using normal phase HPLC

Productivity enhancement with liquid chromatography solutions

Evaluation of Variable Data Independent Acquisition (vDIA) Approach for Nontarget Screening of Veterinary Drugs in Animal Feed

Automated Method Development Utilizing Software-Based Optimization and Direct Instrument Control

A Novel Strategy for the Discovery and Characterization of New Natural Products from Traditional Chinese Medicine by Using Benchtop Orbitrap Mass Spectrometer

Liquid chromatography systems -- the latest LightPipe technology for the UltiMate 3000 Platform

A universal tool for method transfer from HPLC to UHPLC

How does the analysis of biomolecules benefit from UHPLC?

UltiMate 3000 SD HPLC Systems

UltiMate 3000 SD HPLC System

Seamless transfer of a compendial LC method for impurity analysis of chlorhexidine from an UltiMate 3000 Standard HPLC system to a Vanquish Core HPLC system

HPLC and UHPLC method development calculator

HPLC Total Cost of Ownership Calculator

Transfer of a Heart Disease Treatment Analysis from an Agilent 1100 System to an UltiMate 3000 HPLC System

An instrument parameter guide for successful (U)HPLC method transfer

UltiMate 3000 Basic HPLC Systems

UltiMate 3000 Basic Automated HPLC Systems

UltiMate 3000 Semi-Preparative HPLC Systems

Preparative HPLC column method Transfer Tool

EASY-nLC 1200 System

EASY-nLC 1200 System

EASY-nLC 1200 System

Guide to configuring your Thermo Scientific nano LC for mass spectrometric analysis

EASY-Spray best practices

EASY-Spray troubleshooting

Establishing a stable spray on EASY-Spray columns

Improving Retention Time Precision and Chromatography of Early Eluting Peptides with Acetonitrile/Water Blends as Solvent B

Pushing the Limits of Bottom-Up Proteomics with State-Of-The-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes

A novel 75 cm column size gives increased resolution and better sequence coverage

LC System Portfolio

LC Portfolio for LC-MS Applications

EASY-nLC 1200 System – Peak nano LC performance at your fingertips

UltiMate 3000 RSLCnano System

UltiMate 3000 RSLCnano System

UltiMate 3000 RSLCnano System

Guide to configuring your Thermo Scientific nano LC for mass spectrometric analysis

A Highly Sensitive and Robust 150 μm Column to Enable High-Throughput Proteomics

LC/MS Analysis of the Monoclonal Antibody Rituximab Using the Q Exactive Benchtop Orbitrap Mass Spectrometer

Use of a MAbPac RP Capillary Column to Monitor Glycosylation Site Occupancy in Therapeutics

LC System Portfolio

LC Portfolio for LC-MS Applications

UltiMate 3000 RSLCnano System with ProFlow Technology

Thermo Scientific UltiMate 3000 RSLCnano system with ProFlow technology

Capillary-Flow LC-MS: Combining High Sensitivity, Robustness, and Throughput

Capillary-Flow LC-UV Sample Quality Control for Low-Flow LC-MS Based Proteomics

High-Throughput Capillary-Flow LC-MS Proteomics with Maximum MS Utilization

High-Throughput Tandem Capillary-Flow LC-MS for Maximum MS Utilization

Maximize Productivity in Proteome Profiling: Combining Intelligent Tandem nanoLC-MS Methods and a Novel Double-Barrel ESI Source for 100% MS Utilization

Online 2D-nanoLC Hyphenated with High-Resolution Accurate-mass Orbitrap Mass Spectrometry for Comprehensive and Robust Proteome Profiling

Tailored High-Throughput Low-Flow LC-MS Methods for Large Sample Cohort Analysis

Tandem nanoLC-MS: maximum MS utilization for deep-dive proteomic analysis

Transcend LX Multichannel UHPLC Systems

Maximize throughput with multichannel liquid chromatography

LC System Portfolio

Transcend Multichannel LC maintenance guide

Aria MX User Guide

Maximize LC-MS/MS throughput, economy and efficiency using multichannel & on-line sample cleanup technologies

Transcend TLX TurboFlow UHPLC Systems

Maximize throughput with multichannel liquid chromatography

LC System Portfolio

Transcend Multichannel LC

Aria MX User Guide

Maximize LC-MS/MS throughput, economy and efficiency using multichannel & on-line sample cleanup technologies

Component resources

Pumps

UltiMate 3000 Solvent Racks

UltiMate 3000 Pumps

UltiMate 3000 RSLCnano UHPLC System

UltiMate 3000 RSLCnano System with ProFlow Technology

Intelligent UHPLC pump stroke control

Trifluoroacetic acid performance of the Vanquish Flex Binary UHPLC system

Tune your mixing volume for gradient generation to achieve better detection limits

Autosamplers

UltiMate 3000 Well Plate Autosampler

Vanquish Split Sampler

Vanquish Charger

ACC-3000 Autosampler Column Compartment

Vanquish Sample Container Kit

Lifetime stability of size exclusion chromatography columns for protein aggregate analysis

Column Compartments

Vanquish Column Compartments

UltiMate 3000 Thermostatted Column Compartment

ACC-3000 Autosampler Column Compartment

UltiMate 3000 RSLCnano UHPLC System

The Role of Temperature and Column Thermostatting in Liquid Chromatography

Detectors

Charged Aerosol Detector (CAD)

Charged Aerosol Detectors

Vanquish Charged Aerosol Detectors

Corona Veo Charged Aerosol Detector

Peak Scientific Nitrogen Gas Generation System

Customer application Note: Impurity analysis of L-aspartic acid and glycine by HPLC-UV-CAD

Comprehensive analysis of components and degradation products in coolants

Reliable verification and quantification of the presence of extraneous compounds present in a sample, such as impurities, degradation products, or extractables and leachables

HPLC-CAD Excipients applications notebook. Complex substances, universal chromatographic analysis

CAD Bibliography

HPLC-CAD Surfactants and Emulsifiers Applications Notebook

Guidelines for method transfer and optimization—from earlier model Corona detectors to Corona Veo and Vanquish charged aerosol detectors

Charged aerosol detection - factors affecting uniform analyte response

Why use charged aerosol detection with inverse gradient?

Charged aerosol detection – use of the power function and robust calibration practices to achieve the best quantitative

Evaluating HPLC method transfer with charged aerosol detection and inverse gradient

Diode Array Detector (DAD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD)

Vanquish Diode Array Detectors and Multiple Wavelength Detectors

Vanquish Variable Wavelength Detectors

UltiMate 3000 Variable Wavelength Detectors

UltiMate 3000 Diode Array and Multiple Wavelength Detectors

Use of a MAbPac RP capillary column to monitor glycosylation site occupancy in therapeutics

High Salt Gradient Analysis of Post-Translational Modifications - Deamidation Monitoring

Accurate and reliable quantitation of p-toluenesulfonates genotoxic impurities in an active pharmaceutical ingredient by HPLC-UV

Fast Nevirapine Impurity Profiling Using UHPLC-DAD

Simultaneous quantification of nevirapine and low-level impurities

Increased Long-term Stability of Peptide Mapping Using the Vanquish UHPLC System

Liquid chromatography systems -- the latest LightPipe technology for the UltiMate 3000 Platform

Multiple Wavelength Data Acquisition with the Vanquish Variable Wavelength Detector

Boosting Trace Detection Performance with the Vanquish Diode Array Detector and High-Sensitivity LightPipe Flow Cell

Fluorescence Detector (FLD)

Vanquish Fluorescence Detectors

UltiMate 3000 Fluoresecence Detectors

Utilizing the Native Fluorescence of Monoclonal Antibodies for the Sensitive Detection of Charge Variants

Customer Application Note: Validation of routine polycyclic aromatic hydrocarbons analysis in waters

Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors

Fluorescence Method Development Handbook

Combining fluorescence detection with UHPLC: an overview of the technical requirements

Mass Spectrometer (MS), Single Quadrupole

ISQ-EC Single Quadrupole Mass Spectrometer

ISQ-EM Single Quadrupole Mass Spectrometer

Analysis of underivatized amino acids in wines by solid phase extraction and HILIC-MS

A multi-detector platform comprising UV/Vis, charged aerosol, and single quadrupole mass spectrometric detection for comprehensive sample analysis

Detection of oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) in APCI mode with a single quadrupole mass spectrometer

Impurity profiling of the synthetic peptide LL-37 using high-performance liquid chromatography with combined UV and single quadrupole mass spectrometric detection

Oligonucleotide characterization for quality control and increased productivity by single quadrupole mass spectrometer with extended mass range

Identity confirmation and accurate quantitation of a genotoxic impurity in an active pharmaceutical ingredient by UHPLC-UV coupled to a single quadrupole mass detector

Accelerate method scouting with the Thermo Scientifc ISQ EC single quadrupole mass spectrometer and the Thermo Scientific Vanquish Flex Quaternary UHPLC

Achieve confident impurity detection with the Thermo Scientifc ISQ EC single quadrupole mass spectrometer

Achieve confident sysnthesis control with the Thermo Scientifc ISQ EC single quadrupole mass spectrometer

The benefits of adding single quadrupole mass detection to liquid chromatography

Quality control of oligonucleotides with a single quadrupole mass spectrometer

Quick and easy tuning and troubleshooting of ISQ EC and ISQ EM mass spectrometers

Selective analysis of co-eluting isobaric compounds with single quadrupole mass detection