UltiMate 3000 HPLC and UHPLC Systems

Proven systems that are rugged and versatile, with the flexibility to customize

Meet the needs and requirements for your application by selecting from our portfolio of Thermo Scientific UltiMate 3000 HPLC and UHPLC systems. With customizable configurations, these systems provide you with the flexibility to accomplish applications ranging from method development to routine analysis. Alternatively, for routine applications, take advantage of the superior performance, ease-of-use, and dependability offered by the Vanquish Core HPLC systems.

Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.

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Discover which UltiMate 3000 system is right for you

The UltiMate 3000 HPLC series provides excellent chromatographic performance while maintaining easy-to-use, reliable operation. Compatibility with any of our columns offers maximum application flexibility to meet all your separation needs. Regardless of the configuration you choose you will receive a highly integrated setup with optimized fluidic connections, as well as the industry leading easy-to-use Thermo Scientific Chromeleon Chromatography Data System (CDS) software. Our Chromeleon CDS is the preferred software for single-point intelligent control, unique data processing tools, and intelligent run automation functionality. In addition, we offer integration solutions for other CDS packages.

UltiMate 3000 HPLC and UHPLC systems comparison

  Rapid Separation (RS)Standard


System pressure
Retention time precision
Flow range
Low dispersion and high throughput


Pump optionsBinary
Biocompatible options 
Active heating column compartment
Sample cooling    
Column capacity
Viper virtually zero dead volume system connections   
Adjustable gradient delay volume (depending on mixer configuration)  Quaternary Basic:   
Isocratic Basic: n.a.

Application Range

Fraction collection*    
Tandem LC*  
Application switching*  
Inverse gradient for uniform response*  
Automated on-line SPE*  
Automated method development*  
Large volume injection
*Requires additional equipmentThermo Scientific Viper UHPLC Fittings Kits
Order all the UHPLC fittings and capillaries you need for your system or application in a single kit
= Not applicable = Poor = Good = Better = Best

Compatible components for all UltiMate 3000 HPLC and UHPLC systems

Multiple Detector OptionsHPLC and UHPLC ColumnsChromeleon CDS Software
  • Charged aerosol detector
  • Diode array detector
  • Electrochemical detector
  • Fluorescence detector
  • Mass spectrometry detection
  • Multiple wavelength detector
  • Refractive index detector
  • Variable wavelength detector
  • An extensive portfolio of columns
  • In a broad variety of inner diameters, lengths, particle sizes, and chemistries
  • Streamlines your data processing
  • Industry-leading ease of use and intelligent functionality
  • Unique user guidance and hardware automation features designed for UltiMate 3000 systems

Discover the best HPLC systems for your applications and budget

Discover the best HPLC system for your applications and budget

Use these tools to help you decide

HPLC Systems Selection Guide
Use this tool to rank the top HPLC instruments for your applications based on your responses to simple pull-down menus.

HPLC System Total Cost of Ownership Calculator
Use this tool to estimate your cost for purchasing and running Thermo Scientific HPLC and UHPLC systems. You can compare costs between instruments.

LC Method Transfer Calculator
Use this online calculator to transfer methods from HPLC to UHPLC conditions. Calculate the new gradient table, adjust sample volumes and run times if applicable.

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