UltiMate 3000 RSLCnano System

Get outstanding performance and workflow versatility using the Thermo Scientific UltiMate 3000 RSLCnano system. This low-flow liquid chromatography system has a wide flow-pressure footprint with nano-, capillary-, and micro-flow options. Select the nano-flow solution for high sensitivity applications, or choose the robust capillary- and micro-flow solution to balance sensitivity and high throughput performance. Advanced Thermo Scientific ProFlow technology improves nano-flow rate control and results in high retention time precision and gradient reproducibility.


Workflow flexibility


The wide flow range binary gradient pump, additional micro-flow ternary gradient pump, and integrated column compartment with two snap-in switching valves makes the system suitable for virtually any low-flow LC or LC-MS application such as bottom-up and top-down proteomics, or metabolomics. 

Outstanding performance


The integrated micro-flow loading pump enables pre-concentration of samples for ultra-long reproducible gradients. Thermo Scientific ProFlow technology for nano-flow applications provides the highest retention time precision and gradient reproducibility, while the capillary-flow flow meter enables high-sensitivity with high-throughput analyses.



A wide flow rate range and an integrated high-flow micro-flow pump enable quick sample loading, desalting and pre-concentration. Standard and advanced kits for applications such as direct injection, pre-concentration, tandem low-flow LC, or two-dimensional workflows extend this system’s workflow capabilities. 

Seamless LC-MS integration


Dedicated connection kits and LC-MS interfaces are included for straightforward hardware integration with Thermo Scientific EASY-Spray, Thermo Scientific Nanospray Flex, and Thermo Scientific Ion Max sources for maximum ease of use.

Biocompatible, easy and reliable tool-free connections


The fluidic pathway of the UltiMate 3000 RSLCnano UHPLC system is fully biocompatible and fitted with Thermo Scientific nanoViper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software for full LC-MS control


The UltiMate 3000 RSLCnano UHPLC system seamlessly integrates into our Thermo Scientific Chromeleon Chromatography Data System (CDS) as a single point of intelligent control for your HPLC and Thermo Scientific mass spectrometer via Thermo Scientific Standard Instrument Integration (SII) for Xcalibur.


System specifications

The table below provides an overview of the UltiMate 3000 RSLCnano system specifications for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category UltiMate 3000 RSLCnano System
Maximum available column pressure

With nano ProFlow flow meter: 900 bar 

With capillary flow meter: 800 bar 

With micro flow meter: 800 bar 

Flow range, recommended

Nano-flow configuration: 50 nL/min-1.5 µL/min 

Capillary-flow configuration: 0.5-10 µL/min 

Micro-flow configuration: 5-50 µL/min

Pump options (default gradient delay volume) Binary (< 25 nL) and ternary loading pump (220 µL)
Biocompatible Yes
System design Modular
Active flow control  Yes
Injection volume range

Standard: 20 nL–20 µL 

Optional: 20 nL–125 µL

Sample cooling Yes

Sample capacity (standard)

Based on standard 12 mm OD vials (≤1.5 mL) for comparison

3 racks that hold 40 vials each or 3 well plates
Column compartment thermostatting


Forced air

Column compartment pre-heater Not available
Column compartment post-column cooler Not available
Compatible detectors 

Mass Spectrometer

Variable Wavelength Detector