Get outstanding performance and workflow versatility using the Thermo Scientific UltiMate 3000 RSLCnano system. This low-flow liquid chromatography system has a wide flow-pressure footprint with nano-, capillary-, and micro-flow options. Select the nano-flow solution for high sensitivity applications, or choose the robust capillary- and micro-flow solution to balance sensitivity and high throughput performance. Advanced Thermo Scientific ProFlow technology improves nano-flow rate control and results in high retention time precision and gradient reproducibility.
The wide flow range binary gradient pump, additional micro-flow ternary gradient pump, and integrated column compartment with two snap-in switching valves makes the system suitable for virtually any low-flow LC or LC-MS application such as bottom-up and top-down proteomics, or metabolomics.
The integrated micro-flow loading pump enables pre-concentration of samples for ultra-long reproducible gradients. Thermo Scientific ProFlow technology for nano-flow applications provides the highest retention time precision and gradient reproducibility, while the capillary-flow flow meter enables high-sensitivity with high-throughput analyses.
A wide flow rate range and an integrated high-flow micro-flow pump enable quick sample loading, desalting and pre-concentration. Standard and advanced kits for applications such as direct injection, pre-concentration, tandem low-flow LC, or two-dimensional workflows extend this system’s workflow capabilities.
Seamless LC-MS integration
Dedicated connection kits and LC-MS interfaces are included for straightforward hardware integration with Thermo Scientific EASY-Spray, Thermo Scientific Nanospray Flex, and Thermo Scientific Ion Max sources for maximum ease of use.
Biocompatible, easy and reliable tool-free connections
The fluidic pathway of the UltiMate 3000 RSLCnano UHPLC system is fully biocompatible and fitted with Thermo Scientific nanoViper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.
CDS software for full LC-MS control
The UltiMate 3000 RSLCnano UHPLC system seamlessly integrates into our Thermo Scientific Chromeleon Chromatography Data System (CDS) as a single point of intelligent control for your HPLC and Thermo Scientific mass spectrometer via Thermo Scientific Standard Instrument Integration (SII) for Xcalibur.
The table below provides an overview of the UltiMate 3000 RSLCnano system specifications for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.
|Category||UltiMate 3000 RSLCnano System|
|Maximum available column pressure||
With nano ProFlow flow meter: 900 bar
With capillary flow meter: 800 bar
With micro flow meter: 800 bar
|Flow range, recommended||
Nano-flow configuration: 50 nL/min-1.5 µL/min
Capillary-flow configuration: 0.5-10 µL/min
Micro-flow configuration: 5-50 µL/min
|Pump options (default gradient delay volume)||Binary (< 25 nL) and ternary loading pump (220 µL)|
|Active flow control||Yes|
|Injection volume range||
Standard: 20 nL–20 µL
Optional: 20 nL–125 µL
Sample capacity (standard)
Based on standard 12 mm OD vials (≤1.5 mL) for comparison
|3 racks that hold 40 vials each or 3 well plates|
|Column compartment thermostatting||
|Column compartment pre-heater||Not available|
|Column compartment post-column cooler||Not available|
Variable Wavelength Detector
UltiMate 3000 RSLCnano system for nano-, capillary and micro-flow LC and LCMS applications consists of the following modules. Download UltiMate 3000 RSLCnano system specification sheet for more details on each component.
|UltiMate 3000 RSLCnano UHPLC System|
|UltiMate 3000 NCS-3500RS Binary Rapid Separation Nano ProFlow system
(Nano ProFlow Pump, Ternary Loading Pump and Column Compartment)
UltiMate 3000 NCS-3500RS Binary Rapid Separation Capillary Flow system
(Cap-flow Classic Pump, Ternary Loading Pump and Column Compartment)
UltiMate 3000 NCP-3200RS Binary Rapid Separation Nano ProFlow Pump
|UltiMate 3000 WPS-3000TPL RS Rapid Separation Pulled-Loop Nano/Capillary Thermostatted Well Plate Autosampler|
Virtually zero dead volume system connections
|Viper UHPLC Fittings Kits|