Increase your confidence in peak purity using the Thermo Scientific Vanquish Loop Heart-Cut 2D-LC System. This two-dimensional LC system (LC-LC) enables you to collect one or multiple fractions of the first-dimension separation in a sample loop and transfer the cut-out fraction(s) in an automated workflow to a second-dimension separation. Heart-Cut 2D-LC can reveal hidden impurities that co-elute with the analytes of interest by using two complementary column chemistries to gain more separation power. The system is configured using modules from the Vanquish Core HPLC, Vanquish Flex UHPLC or Horizon UHPLC systems, retaining all the performance, robustness and ease of use of the Vanquish LC platform.
Collect one or multiple fractions of the first-dimension separation in a sample loop and transfer the cut-out fraction(s) to a second-dimension column with complementary column chemistry in an automated workflow for more confidence in peak purity.
The modularity of Vanquish LC systems makes it easy to build a system to best suit your application requirements and upgrade a standard HPLC instrument to more advanced 2D-LC configurations when you need to address more challenging analyses.
The integrated LC-LC system in combination with automated workflows, with predefined fractionation and transfer steps, allow for unattended sample analysis while reducing human error and saving time and bench space compared to offline approaches.
The Vanquish Loop Heart-Cut 2D-LC system is fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup
The Vanquish 2D-LC systems seamlessly integrate with our Thermo Scientific Chromeleon Chromatography Data System (CDS) to deliver superior compliance tools, networking capabilities, automation and data processing.
Our loop-based heart-cut 2D-LC system allows you to automate and cut-out fractions(s) of the eluent of the first-dimension (¹D) column into a sample loop to undergo a second separation on a complementary second-dimension (²D) column to resolve difficult or co-eluting peaks.
This flow scheme illustrates a Vanquish Online 2D-LC system for multiple heart-cutting by storing the cut-out fractions in multiple loops before performing a complementary analysis on a second-dimension (²D) column.
The table below provides an overview of the Vanquish Loop Heart-Cut 2D-LC system specifications for standard setups. Not all specifications are listed. Specifications may differ based on the specific system components selected. See detailed Component Specifications for more information.
|Category||Vanquish Loop Heart-Cut 2D-LC Systems|
|System pressure, maximum||700, 1000 or 1500 bar, depends on system configuration|
|Flow range||0-5, 0-8 or 0-10 mL/min, depends on pumps selected|
|Pump options (default delay volume)||
Binary (35, 200 or 400 µL)
Quaternary (679 µL)
Dual (679 µL)
Delay volume depends on pump(s) selected
|Biocompatible||Yes, depends on system configuration|
2–12, chloride concentration up to 1 mol/L
|Touchscreen user interface display compatible (optional)||Yes, depends on system configuration|
|Solvent monitoring system compatible (optional)||Yes, depends on system configuration|
|Customizable gradient delay volume to simplify method transfer||Yes, depends on system configuration|
|SmartInject technology for maximum injection precision and improved column lifetime||Yes|
|Injection volume range||
Standard: 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon)
Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core)
Depends on sampler selected
Depends on sampler selected
Sample capacity (standard)
Based on standard 12 mm OD vials (≤ 1.5 mL)
|4 racks of 54 vials (≤ 1.5mL) each, other rack formats available
+ capacity of 12 x 22.5 mm OD vials (≤ 10 mL) in the carousel
|Sampler extension available||Yes, with Vanquish Charger|
|Column compartment thermostatting||
Yes, 5-120°C, depends on column compartment selected
Still air and forced air
|Column compartment pre-heater||
Active and passive
|Column compartment post-column cooler||
Depends on column compartment selected
Vanquish Charged Aerosol Detector
Vanquish Diode array Detector
Vanquish Fluorescence Detector
Vanquish Multiple Wavelength Detector
Vanquish Variable Wavelength Detector
Refractive Index Detector
Standard Vanquish Loop Heart-Cut 2D-LC systems are made up of the following components. Download the associated specifications sheet for more detail on each component.
|Vanquish Horizon Loop Heart-Cut 2D-LC System||
Vanquish Flex Loop
Heart-Cut 2D-LC System
|Vanquish Core Loop Heart-Cut 2D-LC System|
|2 Vanquish Binary Pump C
2 Vanquish Quaternary Pump C
1 Vanquish Binary Pump C
1 Vanquish Quaternary Pump C
1 Vanquish Dual Pump C
|Vanquish Split Sampler HT||Vanquish Split Sampler FT||Vanquish Split Sampler CT|
Extended sample capacity (optional)
|Vanquish Column Compartment H||Vanquish Column Compartment C|
Virtually zero dead volume system connections
|Viper Fractionation Loop MP35N: 6823.0030 (250 µL), 6823.0029 (50 µL)||
Viper Fractionation Loop MP35N: 6823.0030 (250 µL), 6823.0029 (50 µL)
nanoViper Fractionation Loop, PEEK shielded fused silica
6826.2420 (20µL), 6826.2450 (50µL),
|Viper Fractionation Loop SST: 6830.2418 (200 µL) 6830.2416 (50 µL) 6830.2416 (20 µL)|
|Visit our HPLC and UHPLC Detectors page|