Complete biotherapeutic characterization
Complete characterization includes intact mass analysis, peptide mapping, oligonucleotide analysis, top- and bottom-down analysis or multi-attribute method (MAM) workflows. Access workflows that facilitate comprehensive interpretation and data visualization, allowing you to confidently characterize your biologics with speed and ease. From novel deconvolution algorithms generating complete results to easy-to-understand data visualization tools, Thermo Scientific BioPharma Finder software helps you choose the right path for confident characterization.
Key benefits of the BioPharma Finder software
|Simplify and streamline your workflow analysis using a single software platform||Utilize the accuracy of your high-resolution data for confident intact mass analyses||Know more than ever about your peptide sequence||Completely characterize process and product quality attributes for high throughput QC monitoring|
|Efficiently perform intact analysis, top- and middle-down analysis, HDX and peptide mapping analyses through extensive and efficient workflows.|
From batch processing to in-depth data analyses, workflows guide experts and non-experts to set up, interpret and visualize results, accessing the knowledge contained within any analyzed samples.
|For absolute confidence in your deconvoluted molecular weights in both acidic and native conditions, superior algorithms make the most of high-quality Thermo Scientific Orbitrap mass analyzer data.|
Use all your data, not just a slice, for greater accuracy in deconvolution and relative quantitation of isoforms and drug-to-antibody ratios with the Slide Window algorithm.
|Rapidly process single or multiple data files to identify sequence coverage, expected and unexpected PTMs, disulfide bonds and sequence variants.|
Full MS, MS2 experimental vs. predicted spectra and fragment coverage maps increase confidence in your peptide sequence, with data visualization tools facilitating deeper insights into your peptide maps.
|Confidently discover, identify, quantify and monitor product quality attributes with easy-to-understand data visualization within a single software platform.|
For those requiring compliance tools for GxP compliant monitoring, seamlessly transfer all information from the BioPharma Finder software into the Thermo Scientific Chromeleon 7.2 Chromatography Data System (CDS) for targeted quantitation and new peak detection of peptides and intact proteins.
Learn more about the BioPharma Finder software
The complexity of lifesaving therapies under investigation in the biopharmaceutical industry brings significant challenges from research and discovery, through to development and lot-release and quality control. Some of the toughest tasks for analysts and QC chemists are adequately characterizing these large molecules and subsequently monitoring their quality attributes throughout the manufacturing process.
Find out what it takes to go smoothly from sample to knowledge.
Whether you are analyzing your protein under denaturing conditions or in its native state, default methods and an automated parameter optimizer deliver accurate and confident deconvolution, simplifying your analyses.
BioPharma Finder software can perform batch-to-batch comparisons, or help you confirm the identity of antibody-drug conjugates (ADC). With improved detection of low abundance species using the sliding window algorithm, powerful data visualization tools allow you to gain structural insights into your protein with confidence.
BioPharma Finder software 3.1 or greater gives you the ability to store your intact mass analyses and processed data within a workbook, as well as the ability to edit the contents of each workbook.
As with peptide mapping, analysts increasingly need to perform intact deconvolution under compliant conditions. For laboratories that need to acquire, process and report results within a compliant environment, the Intact Mass Workbook can be seamlessly imported directly into the Chromeleon Chromatography Data System. The ability to maintain compliant workflows by performing method development within the BioPharma Finder software, and then intact mass deconvolution within the BioPharma Finder software or Chromeleon CDS creates a powerful solution serving the entire biopharmaceutical development pipeline.
Novel algorithm for interpretation of LC/MS data of intact proteins. Moving averaged spectrum across the chromatogram (i.e. "Slliding Window" generate deconvoluted spectra for each component, resulting in a single deconvoluted spectrum of all protein components. Supports both high resolution (Xtract) and lower resolution (Respect) data.
Native separations can pose a challenge, especially when it comes to analyzing the resulting data. The novel Sliding Window algorithm ensures that all of your data is used, not just a slice, for deconvolution making sure your DAR calculations are accurate.
An overview of the Sliding Window algorithm, ensuring that you capture all your data, which is critically important for accurate drug to antibody ratio (DAR) calculations, not missing key information on low abundance species.
When you utilize high resolution to obtain the highest quality data and combine that with the ability to operate in multiple fragmentation modes, you need to be sure that you can confidently process and visualize the resulting data.
Simple workflows for sequencing top- and middle-down analyses allow the comparison of multiple data files, if required, as well as providing interactive combined fragmentation coverage maps, which support multiple modes of fragmentation: CID, HCD, ETD, EThcD and UVPD, increasing the amount of sequence coverage for these analyses.
Representation of a middle-down analysis showing chromatographic separation of the light and heavy chains, with excellent mass accuracy observed upon deconvolution. Additionally, the sequence coverage when using both ETD and UVPD fragmentation is illustrated for each of the chains.
Confidently confirm amino acid sequences, with the ability to utilize novel MS2 kinetic fragmentation model predictions and ensure that you capture everything with the identification and location of expected and unknown post-translational modifications. From disulfide bond mapping, to de novo sequencing for unknown components, you can rapidly visualize your data to ensure that you see everything, including low-level impurities and sequence variants, as well as profile relative abundances.
With the BioPharma Finder 3.1 or greater software comes the ability to store the results of your peptide mapping analyses and processed data within a workbook, as well as the ability to edit the contents of each workbook. Additionally, these workbooks can be exported to the Chromeleon Chromatography Data System as part of the multi-attribute method workflow.
With kinetic model predictions for MS2 peptide fragmentation, you can be confident in your peptide sequencing assignments.
During the research and discovery stages of biopharmaceutical characterization, high-resolution mass spectrometry is commonly used to provide an accurate and confident understanding at both the intact and peptide level. However, during lot-release and quality control (QC), a number of release methodologies are performed. Currently, these methods are time consuming, and provide limited information on a single or very few critical quality attributes (CQAs) that need to be monitored.
The multi-attribute method (MAM) is of significant interest to the industry, as it not only allows the implementation of a robust mass spectrometry-based assay for the characterization and release of biotherapeutics, but also offers the potential to reduce the number of ‘traditional’ lot release assays while increasing both product knowledge and the quality and depth of information acquired.
Discovery and early development laboratories operating outside compliance requirements can benefit from straightforward and automated peptide identification, attribute monitoring and new peak detection, as well as relative quantitation for targeted peaks, as defined using the powerful peptide workbook within the BioPharma Finder software. The workbook allows fast screening of large data sets for potential CQA identification, which is verified by a unique MS2 spectra prediction algorithm, isotope distribution and accurate mass information.
For laboratories that need to acquire, process and report results within a compliant environment, the Peptide Workbook can be seamlessly imported directly into the Chromeleon Chromatography Data System. The ability to maintain compliant workflows by performing method development within the BioPharma Finder software, and then intact mass deconvolution within the BioPharma Finder software or Chromeleon CDS creates a powerful solution serving the entire biopharmaceutical development pipeline.
Learn how innovation and monitoring strategies can reduce the number of tests and enhance the methodology of validating impurity.
Representation of the multi-attribute method workflow, where the BioPharma Finder software is used to comprehensively characterize biotherapeutics and determine any potential CQAs to be monitored. The flexibility afforded within the BioPharma Finder software is designed for the discovery environment where compliance is not always necessary. When compliance is required for the routine environment, the knowledge gained can be seamlessly transferred into the Chromeleon CDS for CQA and new peak monitoring.
We're here to help you maximize your free trial of the BioPharma Finder software. Contact us if you would like more information on how to use it, or if you would like to have a representative contact you.
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