LanthaScreen™ fluorescent coregulator peptides for nuclear receptors contain known interaction motifs and are labeled with fluorescein.
| Peptide | 100 μl | Sequence | Description |
|---|---|---|---|
| SRC1-1 | PV4576 | KYSQTSHKLV- QLLTTTAEQQL | Peptide contains the nuclear receptor box1 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC1-2 | PV4578 | LTARHKILHR- LLQEGSPSD | Peptide contains the nuclear receptor box2 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC1-3 | PV4580 | ESKDHQLLR- YLLDKDEKDL | Peptide contains the nuclear receptor box3 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC1-4 | PV4582 | GPQTPQAQQ- KSLLQQLLTE | Peptide contains the nuclear receptor box4 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC2-1 | PV4584 | DSKGQTKLLQ- LLTTKSDQM | Peptide contains the nuclear receptor box1 (LXXLL motif) of SRC-2, member of the p160 coactivator steroid receptor coactivators.2 |
| SRC2-2 | PV4586 | LKEKHKILHRLL- QDSSSPV | Peptide contains the nuclear receptor box2 (LXXLL motif) of SRC-2, member of the p160 coactivator steroid receptor coactivators.2 |
| SRC2-3 | PV4588 | KKKENALLRYLL- DKDDTKD | Peptide contains the nuclear receptor box3 (LXXLL motif) of SRC-2, member of the p160 coactivator steroid receptor coactivators.2 |
| SRC3-1 | PV4590 | ESKGHKKLLQ- LLTCSSDDR | Peptide contains the nuclear receptor box1 (LXXLL motif) of SRC-3, member of the p160 coactivator steroid receptor coactivators.3 |
| SRC3-2 | PV4592 | LQEKHRILHK- LLQNGNSPA | Peptide contains the nuclear receptor box2 (LXXLL motif) of SRC-3, member of the p160 coactivator steroid receptor coactivators.3 |
| SRC3-3 | PV4594 | KKENNALLRY- LLDRDDPSD | Peptide contains the nuclear receptor box3 (LXXLL motif) of SRC-3, member of the p160 coactivator steroid receptor coactivators.3 |
| CBP-1 | PV4596 | AASKHKQLSE- LLRGGSGSS | This peptide contains the LXXLL motif 1 of CREB binding protein and is involved in ligand-dependent nuclear receptor interactions.4 |
| TRAP220/DRIP-1 | PV4598 | KVSQNPILT- SLLQITGNGG | This peptide is the LXXLL motif 1 of the VDR-interacting proteins which interact in a ligand-dependent manner with nuclear receptors.5 |
| TRAP220/DRIP-2 | PV4549 | NTKNHPML- MNLLKDNPAQD | This peptide is the LXXLL motif 2 of the VDR-interacting proteins which interact in a ligand-dependent manner with nuclear receptors.5 |
| RIP140 L6 | PV4600 | SHQKVTLLQ- LLLGHKNEEN | This peptide is derived from the Nuclear receptor interacting protein 1, LxxLL motif 6, known to interact in a ligand-dependent manner with the activation domains of nuclear receptors.6 |
| RIP140 L8 | PV4602 | SFSKNGLLS- RLLRQNQDSY | This peptide is derived from the Nuclear receptor interacting protein 1, LxxLL motif 8, known to interact in a ligand-dependent manner with the activation domains of nuclear receptors.6 |
| PGC1a | PV4421 | EAEEPSLLK- KLLLAPANTQ | This peptide is derived from the PPARg coactivator protein 1a and contains an LXXLL motif.7 |
| PRIP/RAP250 | PV4604 | VTLTSPLLVNL- LQSDISAG | This peptide contains the LXXLL-interaction motif of the PPAR interacting protein, PRIP.4 |
| D22 | PV4386 | LPYEGSLLLK- LLRAPVEEV | Peptide was discovered from random phage display and contains sequences that resemble the known coregulatory proteins, RIP140, PCG-1, DAX-1, and SHP.8 |
| C33 | PV4606 | HVEMHPLLMG- LLMESQWGA | Peptide was discovered from random phage display and contains sequences that resemble the known coregulatory proteins, TRAP220 and RIP140.8 |
| EAB1 | PV4608 | SSNHQSSR- LIELLSR | Peptide was discovered from random phage display to find tamoxifen-induced interactions with ER.9 |
| EA2 | PV4610 | SSKGVLWRM- LAEPVSR | Peptide was discovered from random phage display to find tamoxifen-induced interactions with ER.10 |
| TB3 | PV4614 | SSVASREW- WVRELSR | Peptide was discovered by random phage display during tamoxifen-induced interactions with ER.10 |
| AR(16-34) | PV4616 | SKTYRGAFQ- NLFQSVREVI | This peptide is the N-terminal sequence of AR which mediates a ligand-induced intradomain interaction with the ligand binding domain.11 |
| ARA70 | PV4618 | SRETSEKFKL- LFQSYNVND | This peptide is the FxxLF motif of the AR-specific coactivator, ARA70.11 |
| D11 FxxLF | PV4382 | VESGSSRFM- QLFMANDLLT | This random phage display peptide had been modified to contain the AR-specific FxxLF interaction motif.11 |
| SMRT ID1 | PV4620 | GHQRVVTLA- QHISEVITQDYTRH | This peptide contains the interaction motif 1 of the corepressor protein, Silencing Mediator for Retinoid and Thyroid-hormone receptors.12 |
| SMRT ID2 | PV4423 | HASTNMGLEA- IIRKALMGKYDQW | This peptide contains the interaction motif 1 of the corepressor protein, Silencing Mediator for Retinoid and Thyroid-hormone receptors.12 |
| NCoR ID1 | PV4622 | RTHLITLADH- ICQIITQDFARN | This peptide contains motif 1 of the Nuclear CoRepressor protein.13 |
| NCoR ID2 | PV4624 | DPASNLGLEDII- RKALMGSFDDK | This peptide contains motif 2 of the Nuclear CoRepressor protein.13 |
References
- Onate SA, Tsai SY, Tsai MJ, O’Malley BW (1995) Science 270:1354-1357
- Takeshita A, Yen PM, Misiti S, Cardona GR, Liu Y, Chin WW (1996) Endocrinology 137: 3594-3597.
- Anzick SL, Kononen J, Walker RL, Azorsa DO, Tanner MM, Guan XY, Sauter G, Kallioniemi OP, Trent JM, Meltzer PS (1997) Science 277:965-968.
- Caira F, Antonson P, Pelto-Huikko M, Treuter E, Gustafsson J-A (2000) J Biol Chem 275:5308-5317.
- Rachez C, Gamble M, Chang C-PB, Atkins GB, Lazar MA, Freedman LP (2000) Mol Cell Biol 20:2718-2726.
- Cavailles V, Dauvois S, L’Horset F, Lopez F, Hoare S, Kushner PJ, Parker MG (1995) EMBO J. 14:3741-3751.
- Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM (1998) Cell 92: 829-839
- Chang CY, Norris JD, Grøn H, Paige LA, Hamilton PT, Kenan DJ, Fowlkes D, McDonnell DP (1999) Mol Cell Biol 19:8226-8239.
- Norris JD, Paige LA, Christensen DJ, Chang CY, Huacani MR, Fan D, Hamilton PT, Fowlkes DM, McDonnell DP (1999) Science 285:744-746.
- Heldring N, Nilsson M, Buehrer B, Trueter E, Gustafsson J-A (2004) Mol Cell Biol 24:3445-3459.
- He B, Minges JT, Lee LW, Wilson EM (2002) J Biol Chem 277:10226-10235.
- Chen JD, Evans RM (1995) Nature 377:454-457.
- Perissi V, Staszewski LM, McInerney EM, Kurokawa R, Krones A, Rose DW, Lambert MH, Milburn MV, Glass CK, Rosenfeld MG (1999) Genes Dev 13:3198-3208.
| Peptide | 100 μl | Sequence | Description |
|---|---|---|---|
| SRC1-1 | PV4576 | KYSQTSHKLV- QLLTTTAEQQL | Peptide contains the nuclear receptor box1 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC1-2 | PV4578 | LTARHKILHR- LLQEGSPSD | Peptide contains the nuclear receptor box2 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC1-3 | PV4580 | ESKDHQLLR- YLLDKDEKDL | Peptide contains the nuclear receptor box3 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC1-4 | PV4582 | GPQTPQAQQ- KSLLQQLLTE | Peptide contains the nuclear receptor box4 (LXXLL motif) of SRC-1, member of the p160 coactivator steroid receptor coactivators.1 |
| SRC2-1 | PV4584 | DSKGQTKLLQ- LLTTKSDQM | Peptide contains the nuclear receptor box1 (LXXLL motif) of SRC-2, member of the p160 coactivator steroid receptor coactivators.2 |
| SRC2-2 | PV4586 | LKEKHKILHRLL- QDSSSPV | Peptide contains the nuclear receptor box2 (LXXLL motif) of SRC-2, member of the p160 coactivator steroid receptor coactivators.2 |
| SRC2-3 | PV4588 | KKKENALLRYLL- DKDDTKD | Peptide contains the nuclear receptor box3 (LXXLL motif) of SRC-2, member of the p160 coactivator steroid receptor coactivators.2 |
| SRC3-1 | PV4590 | ESKGHKKLLQ- LLTCSSDDR | Peptide contains the nuclear receptor box1 (LXXLL motif) of SRC-3, member of the p160 coactivator steroid receptor coactivators.3 |
| SRC3-2 | PV4592 | LQEKHRILHK- LLQNGNSPA | Peptide contains the nuclear receptor box2 (LXXLL motif) of SRC-3, member of the p160 coactivator steroid receptor coactivators.3 |
| SRC3-3 | PV4594 | KKENNALLRY- LLDRDDPSD | Peptide contains the nuclear receptor box3 (LXXLL motif) of SRC-3, member of the p160 coactivator steroid receptor coactivators.3 |
| CBP-1 | PV4596 | AASKHKQLSE- LLRGGSGSS | This peptide contains the LXXLL motif 1 of CREB binding protein and is involved in ligand-dependent nuclear receptor interactions.4 |
| TRAP220/DRIP-1 | PV4598 | KVSQNPILT- SLLQITGNGG | This peptide is the LXXLL motif 1 of the VDR-interacting proteins which interact in a ligand-dependent manner with nuclear receptors.5 |
| TRAP220/DRIP-2 | PV4549 | NTKNHPML- MNLLKDNPAQD | This peptide is the LXXLL motif 2 of the VDR-interacting proteins which interact in a ligand-dependent manner with nuclear receptors.5 |
| RIP140 L6 | PV4600 | SHQKVTLLQ- LLLGHKNEEN | This peptide is derived from the Nuclear receptor interacting protein 1, LxxLL motif 6, known to interact in a ligand-dependent manner with the activation domains of nuclear receptors.6 |
| RIP140 L8 | PV4602 | SFSKNGLLS- RLLRQNQDSY | This peptide is derived from the Nuclear receptor interacting protein 1, LxxLL motif 8, known to interact in a ligand-dependent manner with the activation domains of nuclear receptors.6 |
| PGC1a | PV4421 | EAEEPSLLK- KLLLAPANTQ | This peptide is derived from the PPARg coactivator protein 1a and contains an LXXLL motif.7 |
| PRIP/RAP250 | PV4604 | VTLTSPLLVNL- LQSDISAG | This peptide contains the LXXLL-interaction motif of the PPAR interacting protein, PRIP.4 |
| D22 | PV4386 | LPYEGSLLLK- LLRAPVEEV | Peptide was discovered from random phage display and contains sequences that resemble the known coregulatory proteins, RIP140, PCG-1, DAX-1, and SHP.8 |
| C33 | PV4606 | HVEMHPLLMG- LLMESQWGA | Peptide was discovered from random phage display and contains sequences that resemble the known coregulatory proteins, TRAP220 and RIP140.8 |
| EAB1 | PV4608 | SSNHQSSR- LIELLSR | Peptide was discovered from random phage display to find tamoxifen-induced interactions with ER.9 |
| EA2 | PV4610 | SSKGVLWRM- LAEPVSR | Peptide was discovered from random phage display to find tamoxifen-induced interactions with ER.10 |
| TB3 | PV4614 | SSVASREW- WVRELSR | Peptide was discovered by random phage display during tamoxifen-induced interactions with ER.10 |
| AR(16-34) | PV4616 | SKTYRGAFQ- NLFQSVREVI | This peptide is the N-terminal sequence of AR which mediates a ligand-induced intradomain interaction with the ligand binding domain.11 |
| ARA70 | PV4618 | SRETSEKFKL- LFQSYNVND | This peptide is the FxxLF motif of the AR-specific coactivator, ARA70.11 |
| D11 FxxLF | PV4382 | VESGSSRFM- QLFMANDLLT | This random phage display peptide had been modified to contain the AR-specific FxxLF interaction motif.11 |
| SMRT ID1 | PV4620 | GHQRVVTLA- QHISEVITQDYTRH | This peptide contains the interaction motif 1 of the corepressor protein, Silencing Mediator for Retinoid and Thyroid-hormone receptors.12 |
| SMRT ID2 | PV4423 | HASTNMGLEA- IIRKALMGKYDQW | This peptide contains the interaction motif 1 of the corepressor protein, Silencing Mediator for Retinoid and Thyroid-hormone receptors.12 |
| NCoR ID1 | PV4622 | RTHLITLADH- ICQIITQDFARN | This peptide contains motif 1 of the Nuclear CoRepressor protein.13 |
| NCoR ID2 | PV4624 | DPASNLGLEDII- RKALMGSFDDK | This peptide contains motif 2 of the Nuclear CoRepressor protein.13 |
References
- Onate SA, Tsai SY, Tsai MJ, O’Malley BW (1995) Science 270:1354-1357
- Takeshita A, Yen PM, Misiti S, Cardona GR, Liu Y, Chin WW (1996) Endocrinology 137: 3594-3597.
- Anzick SL, Kononen J, Walker RL, Azorsa DO, Tanner MM, Guan XY, Sauter G, Kallioniemi OP, Trent JM, Meltzer PS (1997) Science 277:965-968.
- Caira F, Antonson P, Pelto-Huikko M, Treuter E, Gustafsson J-A (2000) J Biol Chem 275:5308-5317.
- Rachez C, Gamble M, Chang C-PB, Atkins GB, Lazar MA, Freedman LP (2000) Mol Cell Biol 20:2718-2726.
- Cavailles V, Dauvois S, L’Horset F, Lopez F, Hoare S, Kushner PJ, Parker MG (1995) EMBO J. 14:3741-3751.
- Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM (1998) Cell 92: 829-839
- Chang CY, Norris JD, Grøn H, Paige LA, Hamilton PT, Kenan DJ, Fowlkes D, McDonnell DP (1999) Mol Cell Biol 19:8226-8239.
- Norris JD, Paige LA, Christensen DJ, Chang CY, Huacani MR, Fan D, Hamilton PT, Fowlkes DM, McDonnell DP (1999) Science 285:744-746.
- Heldring N, Nilsson M, Buehrer B, Trueter E, Gustafsson J-A (2004) Mol Cell Biol 24:3445-3459.
- He B, Minges JT, Lee LW, Wilson EM (2002) J Biol Chem 277:10226-10235.
- Chen JD, Evans RM (1995) Nature 377:454-457.
- Perissi V, Staszewski LM, McInerney EM, Kurokawa R, Krones A, Rose DW, Lambert MH, Milburn MV, Glass CK, Rosenfeld MG (1999) Genes Dev 13:3198-3208.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
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