Every PolarScreen™ Competitive Binding Assay for Nuclear Receptors includes protein, a proprietary fluorescent Fluormone™ ligand, and an optimized buffer system. When the NR binds to the Fluormone™ ligand, the resulting complex yields a high polarization value. If the test compound displaces the Fluormone™ ligand from the complex, the polarization value is lowered. Since this occurs only in the presence of a test compound, the shift in polarization value enables you to accurately and conveniently determine relative affinity of a test compound for the NR.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.