Effectively analyze signal transduction pathways of biological relevance.
Our CellSensor Cell Lines can be used to:
- Screen for agonists/antagonists within a signaling pathway
- Identify inhibitors of a specific protein target in a signaling pathway
- Counterscreen and profile for compound selectivity
View our CellSensor Cell Line Validation Table to find your target and start using the largest collection of fully validated pathway cell lines for your primary and secondary screening today.
CellSensor Cell Lines use GeneBLAzer® beta-lactamase reporter technology to provide you with a rapid and sensitive method of analyzing signal transduction pathways exposed to drug candidates or other stimuli. These cell lines measure compound potency and selectivity and allow for analysis, in a high-throughput manner, of key pathways activated or down-regulated by compounds.
Many intracellular signaling pathways converge at the transcriptional level, where transcription factors bind to DNA enhancer elements that regulate downstream transcription. We have used lentiviral vectors to produce a series of stable cell lines containing various response elements upstream of the beta-lactamase reporter gene.
When pathways leading to specific enhancer elements are activated in these cell lines, beta-lactamase is produced, which can then be detected in a homogeneous assay with our cell-permeable, ratiometric fluorescent LiveBLAzer-FRET B/G substrate (Figure 1).
There are two critical instrumentation features required for CellSensor readout—bottom read capability and compatible filter sets. Invitrogen has validated several instruments for use with our CellSensor Cell Lines when used with our LiveBLAzer-FRET B/G substrate.
For instrumentation set-up help or a list of validated instruments, please email us at firstname.lastname@example.org.
For Research Use Only. Not for use in diagnostic procedures.