Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Invitrogen Qubit Fluorometers were developed to work optimally with the Qubit Assays. Together they quickly and specifically quantify DNA, RNA, and protein. It is easier than ever to determine if you have sufficient nucleic acid or protein for your experiment.
Get accurate data in a few seconds with touch screen–controlled operation and your choice of assays.
Get the one-of-a-kind 8-sample fluorometer for flexible throughput plus all the benefits of the Qubit 4 Fluorometer.
Find reagent kits for DNA, RNA, and protein quantification, even for dilute samples.
Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. These fluorescent dyes emit only when bound to the target molecules, even at low concentrations (Figure 1). Both the Qubit 4 Fluorometer and the Qubit Flex Fluorometer are orders of magnitude more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA (Figure 2). With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.
Figure 1. Performance of the Qubit dsDNA HS Assay. The Invitrogen Qubit dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.
Figure 2. Qubit platforms give sensitive, accurate and precise measurements compared to UV absorbance instruments. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/μL were assayed using the Qubit dsDNA HS Assay on the Qubit Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a microvolume spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit assay tubes.
Download the latest software and MyQubit applications, read FAQs, citations, and application notes
See the full selection of Invitrogen Qubit assays to accurately detect DNA, RNA and protein using Qubit fluorometers.
Learn how our powerful portfolio of benchtop instruments can help to simplify tedious tasks, keep you safe in the lab, and enable more accurate results so you can focus on achieving your next breakthrough.
For Research Use Only. Not for use in diagnostic procedures.