Qubit Fluorometric Quantification

Sensitive, accurate biomolecule quantification

Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. These fluorescent dyes emit only when bound to the target molecules, even at low concentrations (Figure 1). Both the Qubit 4 Fluorometer and the Qubit Flex Fluorometer are orders of magnitude more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA (Figure 2). With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Technical resources

Download the latest software and MyQubit applications, read FAQs, citations, and application notes

The Qubit quantification platform continues to evolve with you, offering:

• Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance
• More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA
• Uses as little as 1 μL of sample, even with very dilute samples
• New integrated reagent calculator to quickly generate working solution calculations
• Flexible options for exporting results: Wi-Fi, USB drive, or direct connection with a USB cable
• Highly accurate Qubit Flex Fluorometer increases throughput capacity, measuring 8-samples at once