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Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. These fluorescent dyes emit only when bound to the target molecules, even at low concentrations (Figure 1). Both the Qubit 4 Fluorometer and the Qubit Flex Fluorometer are orders of magnitude more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA (Figure 2). With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.
Figure 1. Performance of the Qubit dsDNA HS Assay. The Invitrogen Qubit dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.
Figure 2. Qubit platforms give sensitive, accurate and precise measurements compared to UV absorbance instruments. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/μL were assayed using the Qubit dsDNA HS Assay on the Qubit Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a microvolume spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit assay tubes.
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For Research Use Only. Not for use in diagnostic procedures.