Anion exchange chromatography is a form of ion exchange chromatography where a negatively charged biomolecule binds to a positively charged resin.
POROS resinType of AEX resinSurface chemistryBSA binding capacity (mg/mL)Pore size
D50WeakDimethylaminopropyl901,050 Å
PI50WeakPolyethyleneimine (mixed amine)802,000 Å
HQ50StrongQuaternized polyethyleneimine (mixed amine)752,000 Å
XQStrongFully quaternized amine>1401,100 Å

These AEX resins can be used for a variety of applications:

  • Bind/elute: Protein, virus, and plasmid DNA purification
  • Flow/through: Trace impurity removal by binding impurities (DNA, viruses, HCP, aggregates, and endotoxins)

Figure 1. With features of high capacity, high resolution, and high salt tolerance, the anion exchange resin contributes optimized features for the best results.

Product ordering

POROS Anion Exchange Resins (POROS AEX resins) are designed for high-dynamic binding capacity over a range of pH and conductivity conditions, thereby increasing process development flexibility and manufacturing throughput. The 50 μm particle size provides superior resolution for unprecedented impurity clearance independent of scale and flow rate (Figure 1).

POROS AEX resins are 50 μm, rigid, polymeric, ion‑exchange chromatography resins that can be used for the chromatography of biomolecules including recombinant proteins, monoclonal antibodies, DNA, viruses, and peptides. The resin backbone consists of cross-linked poly[styrene divinylbenzene]. A polyhydroxyl surface coating provides low nonspecific binding and different surface chemistries to provide four anion exchanger resins that are unique to the market.

Platform purification approaches for the production of complex biologics

Process development scientists are looking for new tools that will clear even more impurities in a single step while maintaining high recoveries and process flexibility. Watch this video to learn how Thermo Scientific POROS Ion Exchange chromatography resins address purification challenges of today’s biomolecules.  

Application Notes

Bioprocessing resources

Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing: