DMEM/F-12 Dulbecco’s Modified Eagle Medium F-12

Gibco Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) is formulated for superior quality, delivers greater reliability, consistency and improved control in mammalian cell culture.

DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F-12, this formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

We offer a variety of DMEM/F-12 modifications for a range of cell culture applications.

NEW Gibco Human Plasma-like Medium

Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma.

  • Physiologically relevant - designed to resemble the natural cellular environment found in the body formulated with more than 60 polar metabolites and salt concentrations
  • Peer reviewed – extensive research publications completed using HPLM formulation

Discover Human Plasma-like Medium

Choose the right DMEM/F-12

Select the right Gibco DMEM/F-12 for your cell culture. Browse the catalog and you’ll be able to narrow the list according to:

  • Glutamine/GlutaMAX supplementation
  • Phenol red indicator
  • Liquid vs powder format
  • Serum supplementation
  • Sodium pyruvate additive
  • Application

Specialty DMEM/F12 formulations

Take your cell culture to the next level with enhanced formulations of your everyday favorites.

  • Reduced-serum media to lower cost of serum by 50–90%
  • Greater consistency with fewer lot-to-lot serum changes

Choose Advanced DMEM/F12

  • Pre-supplemented with stable form of L-glutamine to prevent ammonia buildup
  • High viability for long-term cultures

Choose DMEM/F12 with GlutaMAX

Need a custom DMEM/F12 formulation? Customize your DMEM/F-12 media online

Taking your work to the clinic? Be sure to check out the CTS KnockOut DMEM/F-12

cell culture media icon GMP manufacturing

Gibco cell culture products are manufactured in facilities that are compliant with current good manufacturing practices (GMP) for medical devices and adheres to a robust quality management system.

Learn more about GMP manufacturing

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We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.

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