Invitrogen GeneArt Gene Synthesis

GeneArt Gene Synthesis is a reliable and cost-effective method for obtaining customized DNA constructs with 100% sequence accuracy. Our GeneOptimizer algorithm utilizes a multifactorial approach (up to 20 parameters) beyond just traditional codon optimization to significantly improve protein expression when it comes wild-type protein.

The GeneArt Instant Designer ordering tool provides a powerful yet simple approach to ordering with both Prime and Value Gene Synthesis Services to tackle everything from simple to complex sequences.

Download GeneArt Services Resource Guide

Priority for SARS-CoV-2 projects

As part of Thermo Fisher Scientific's commitment to help accelerate discovery for potential vaccine development GeneArt Gene Synthesis Services are automatically prioritizing projects for our Express service when it confirms that the sequences are related to SARS-CoV-2. There is no need to specify Express Service; we'll automatically detect and prioritize the constructs are related to SARS-CoV-2.

For Email orders simply add "Urgent: Coronavirus" in the subject line, and we'll automatically prioritize.

Option 1: Order your genes with GeneArt Instant Designer

Feature Highlights

  • Fast and intuitive with real-time pricing
  • Value Gene pricing options
  • Express Cloning (including Gateway and TOPO)
  • Bulk upload of DNA & Protein sequences
  • Real-time optimization
  • Midi-Giga plasmid prep scales

Production times | Compare services | Quick reference guide | Available vectors

Order now

Looking to order GeneArt Strings DNA Fragments? Order here

Option 2: Classic GeneArt Gene Synthesis Portal

  • Upload your own custom vectors
  • Wide selection of vectors
  • Classic interface
  • Access previously saved projects ordered using the classic portal

For a limited time, save up to 30% on gene synthesis-no promotion code is required. Simply click the order button to get started and save.

Option 3: Email Ordering

Option 4: GeneArt API

Introducing Value and Prime Gene Synthesis Services

With our new Instant Designer gene synthesis tool, we’ve introduced 2 pricing tiers for even better value. Based on your sequence criteria, you’ll have the option to choose within the tool and be guided at each step of the way. Below is a guide to which service level to choose.

 Classic GeneArt Gene Synthesis PortalGeneArt Instant Designer
 Gene Synthesis ServicesValue Gene Sythesis ServicesPrime Gene Synthesis Services
Available Vectors
  • Standard GeneArt delivery vector with ampicillin or kanamycin resistance markers (pMX, pMA, and pMK)
  • >30 available vectors
  • Upload your own vector
  • Standard GeneArt delivery vector with ampicillin or kanamycin resistance markers (pMX, pMA, and pMK)

Express Cloning Vectors:

  • Standard GeneArt delivery vector with ampicillin or kanamycin resistance markers (pMX, pMA, and pMK)

Express Cloning Vectors:

Express cloningYesYesYes
Order methodOffline and onlineOnline onlyOffline and online
Sequence complexityAvailable for complex and non-complex sequencesNon-Complex Sequences (GC content 20–80%, uncomplicated secondary structure and without direct/indirect repeats, or long polypurine/polypyrimidine runs)Available for complex and non-complex sequences
Supported sequence length (bp)100–9,000200–5,000100–12,000
QC sequencing method
  • Sanger Sequencing
  • Next Gen Sequencing
  • Next Gen Sequencing
  • Sanger Sequencing
  • Next Gen Sequencing
Add-on services or upgrades
  • Express, SuperSPEED
  • Sanger Sequencing
  • TSE-Free
Not applicable
  • Express, SuperSPEED
  • Sanger Sequencing
Standard production timeStarting from 7 days*Starting from 9 daysStarting from 7 days*

* Express and SuperSPEED are available for faster service. For a more detailed see the table below.

Order with confidence

Like all GeneArt Gene Synthesis services, our standard gene synthesis includes the following components to help assure that your construct meets all of your research, quality, and documentation requirements.

  • Configure your project on your own in our online portal
  • Fast and easy project design
  • Instant overview of project history
  • Complete documentation

If you are new to GeneArt Gene Synthesis, check out our online ordering quick start guide and our gene synthesis tutorials.

GeneArt Gene Synthesis production times

It is important to note that genes are made-to-order products and can show variations in producibility, depending on the complexity of the DNA sequence. Typical production times are met for > 90% of genes produced. Production time is now faster by 2 days for genes up to 7 kb and by 9 days for genes between 7–9 kb. Production time is the number of business days required to synthesize genes in our manufacturing facility. Delivery time is in addition to production time and depends on the destination of the shipment.

range (bp)
Prime Gene SynthesisValue Gene Synthesis
200–1,2007-96-75Starting from 9
1,201–2,0009-128-106-7 (up to 1,800bp)13
3,001–5,000Starting from 17Starting from 15N/AStarting from 20
5,001–7,000Starting from 22Starting from 20N/AN/A
7,001–9,000Starting from 22Starting from 20N/AN/A
9,001–12,000Starting from 27Starting from 25N/AN/A

* Valid for standard gene synthesis (non-complex, GC content 20-80%, repetitions, secondary structures). For complex sequences, production times will be longer and these will be communicated prior to production.
** Subject to sequence assessment. Order must be placed by 3:00 p.m. CET.
Please note: Rarely, requested sequences are found to be toxic and/or genetically unstable. These production times are only valid for nontoxic sequences that are genetically stable in E. coli.

(Sub) genome and long gene synthesis ordering

For researchers who need long custom DNA constructs for pharmaceutical development (e.g. viral genomes) or for metabolic engineering, pathway engineering, or synthetic biology, we offer a fast, reliable, and economic solution—our proprietary and completely in-house GeneArt DNA synthesis and assembly services.

  • High-throughput  gene synthesis with a capacity of >8.5 Mbp per month (fast and reliable production of DNA pieces for assembly)
  • Expert design and assembly technologies and process expertise for the assembly of constructs from 10 kb to several hundred kilobases, with timelines starting from 25 business days
  • Robust technologies to build DNA constructs with highest complexity
  • Sanger sequencing and next-generation sequencing offer flexible solutions for sequence verification and final QC
  • Proven track record of reliable and timely production for long and complex constructs, with dozens of constructs built each year
  • Proprietary technologies to build high-quality gene fragments up to 3 kb, and assembly kits that enable do-it-yourself construction and assembly

Learn more about (Sub) genome size and long gene synthesis

Why choose GeneArt Gene Synthesis

  • Flexible, custom process: Obtain the exact sequence you need in the vector of your choice—including challenging, complex constructs, and custom vectors.
  • Maximized protein yield: Optional sequence processing with GeneOptimizer software generates sequence variants with enhanced mRNA stability and translational efficiency.
  • Rapid synthesis and assembly: Standard turnaround times start at 10 business days (accelerated options available).
  • Economical: The cost of the GeneArt Gene Synthesis service rivals costs that would be incurred to purchase the oligonucleotides and molecular biology reagents required for gene synthesis, and outsourcing your gene synthesis and cloning means you can focus on your research.
  • Excellent accuracy: All custom synthesized genes are sequenced as part of our ISO 9001:2015–certified quality management system; we only ship constructs with exact sequence agreement.
  • Multiple options: We can synthesize ready-to-clone linear fragments, synthetic genes in circular plasmids, complete vectors, and more. Compare all gene synthesis options 
  • "GeneArt it": Save time and labor by outsourcing your gene synthesis and cloning so you can focus on your research.

Gene synthesis is a fast, simple alternative to traditional cloning

Whether you have limited cloning experience or simply want to save time, the GeneArt Gene Synthesis service helps you move your ideas from the planning stage to the laboratory more quickly. Benefit from our experience in successfully producing over 180,000 constructs for customers as diverse as large pharmaceutical companies, biotechnology start-ups, and basic research institutions. The comparison shown below highlights the time and effort saved compared to traditional cloning.

Why choose GeneArt Gene Synthesis

GeneArt Gene Synthesis process

GeneArt gene synthesis process diagram 

Submitted DNA sequences are designed by our team of highly skilled scientists to include/exclude restriction enzyme sites and translation elements as requested, and are optionally optimized using our GeneOptimizer software for maximum protein production.

Oligonucleotides synthesized in-house, with maximum sequence accuracy, serve as the building blocks for custom gene synthesis.

Genes are assembled using our GeneAssembler process, which facilitates high-throughput gene assembly on an industrial scale for rapid order fulfilment. Using our GeneObserver online tracking system, you can access detailed information 24 hours a day about your order as it progresses through our synthesis process.

Constructs are cloned into one of our standard cloning vectors, or you can specify your own custom vector by adding a subcloning service (additional fees will apply).

All constructs are sequence-verified; only those that match the requested sequence 100% are shipped. The DNA is delivered as 5 µg lyophilized plasmid DNA in microcentrifuge tube.

Quality of every gene construct is controlled as part of our ISO 9001:2015-certified quality management system, which helps ensure reliability and reproducibility. A detailed synthesis report including plasmid map, sequence alignment, and restriction map is provided with each construct.

Up to a 100-fold increase in expression

The GeneOptimizer process has been shown to help deliver large increases in protein yield via a combination of factors that stabilize mRNA and maximize translation efficacy (see figure). Efficacy has been proven in a large comparative gene expression study (Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression Fath et al., 2011 PLoS ONE).

Comparative expression analysis of wild type vs. an optimized human gene

Comparative expression analysis of wild type vs. an optimized human gene. Western blot analysis using α-His antibody.Expression levels of 2 independent transfections per wild type and optimized construct are displayed, showing a 3-fold difference in expression in this particular analysis. Standardization is based on endogenous protein.

Save time with the new express cloning service

Save time with the new express cloning service

Using an alternative production procedure with pre-prepared expression vectors, we can clone your GeneArt synthesized genes directly into selected expression plasmids, saving 4–5 business days compared to the standard workflow with cloning into pMx series vector first.

Turnaround time for expression-ready genes starts at 11 business days (depending on the length of your gene). Adding the gene synthesis express upgrade cuts an additional 2 days, so it is possible to get a turnaround time of 9 business days for your cloned, expression-ready gene.

Learn more about our express cloning service

For Research Use Only. Not for use in diagnostic procedures.