Ribonuclease (RNase) Inhibitors

RNase inhibitors (ribonuclease inhibitors) are recombinant enzymes used to inhibit RNase activity during your experiments. RNase inhibitors are commonly used as a precautionary measure in enzymatic manipulations of RNA to inhibit and control for such contaminants. Small amounts of ribonucleases can co-purify with isolated RNA and compromise downstream applications. Such contamination can also be introduced via tips, tubes, and other reagents used in procedures.

RNase inhibitor products

We offer several nuclease inhibitors: Invitrogen SUPERase In RNase Inhibitor, RNaseOUT Recombinant Ribonuclease Inhibitor, RNAsecure RNase Inactivation Reagent, and RNase Inhibitor. See which inhibitor or reagent is right for your research.

Nuclease inhibitorSUPERase• In RNase Inhibitor (20 U⁄μL)
  • Robust protein-based RNase inhibitor
  • Inhibits RNases A, B, C, 1, and T1
  • Active at up to 65°C and from pH 5.5 to 8.5
  • No DTT requirements
  • Requires no DTT, but is functional in DTT concentrations as high as 200 mM DTT
  • Retains activity in multiple freeze-thaw cycles
RNaseOUT Recombinant Ribonuclease Inhibitor
  • Noncompetitive, recombinant, protein-based RNase inhibitor
  • Inhibits RNase A, B, and C
RNAsecure Reagent
  • A nonenzymatic reagent that irreversibly inactivates RNases in solutions that
    • Cannot be treated with DEPC
    • Cannot be autoclaved
    • Contain enzymatic reactions prior to adding the enzyme
  • Does not require post-treatment autoclaving
  • Reheating after initial treatment reactivates the RNase-destroying agent to minimize new contamination
RNase Inhibitor
  • Recombinant ribonuclease inhibitor
  • Inhibits RNases A, B, and C
  • Does not inhibit RNase I, T1, T2, H, U1, U2, or CL3
  • Requires a minimum of 1 mM DTT
  • Effective pH range: 5.0–8.0
RNase Inhibitor (Cloned) 40 U⁄μL
  • Cloned version of RNase inhibitor