High quality gRNAs for any CRISPR application

When using the CRISPR-Cas9 system to knockout gene expression or knock-in a specific mutation, the design, production, and delivery of high quality gRNAs are critical to achieving a successful result. The scientists at Thermo Fisher Scientific  have developed multiple CRISPR gRNA solutions to help you realize your goals and develop high impact models to move your research forward. Whether you need transfection-ready gRNAs for use with Invitrogen TrueCut Cas9 Protein v2 or you need to harness lentivirus to deliver your editing tools to hard to transfect or primary cells, we have you covered. Use our selection guide below to find the right gRNAs for your experiment.

Available formats

TrueGuide Synthetic gRNA

LentiArray Lentiviral gRNA

IVT gRNA Custom Service
OverviewChoose from our catalog of predesigned gRNAs or upload your sequence to our Invitrogen TrueGuide gRNA Ordering ToolChoose your gRNA design from our catalog of gRNAs packaged as ready to use lentivirus.Utilize our in-house expertise and have our GeneArt custom services team design and synthesize ready to transfect gRNAs for you or design them yourselves using our design tool.
FormatSynthetic gRNA oligoLentivirus lentiviral gRNAIn vitro (IVT) gRNA
ApplicationKnockout or knock-inKnockout or library screeningKnockout or knock-in
SpeciesAll speciesHumanAll species
Delivery methodLipid-mediated transfection or electroporationLentiviral deliveryLipid-mediated transfection or electroporation
Recommended Cas9 FormatInvitrogen TrueCut Cas9 Protein v2Invitrogen LentiArray Cas9 LentivirusInvitrogen TrueCut Cas9 Protein v2
ControlsPositive and negative controls availablePositive and negative controls availableAvailable upon request
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CRISPR Controls

High-quality controls play an integral role in the successful development and performance of high-throughput screens. Don't forget to include controls to help you optimize delivery conditions, maximize editing efficiency, and establish hit selection criteria. Learn more

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