There are two types of TaqMan chemistry options available for miRNA profiling studies:
- The first generation TaqMan micoRNA Assays use an miRNA-specific reverse transcription (RT) stem loop primer to generate cDNA for the specific miRNA targets of interest.
- In contrast, the new TaqMan Advanced miRNA chemistry uses an universal RT primer to reverse transcribe all of the miRNAs in the sample. The TaqMan Advanced miRNA chemistry is the preferred option for large profiling studies as it allows for a simplified and specific workflow for profiling large numbers of miRNAs.
|TaqMan MicroRNA Assays||TaqMan Advanced miRNA Assays|
|Description||TaqMan MicroRNA Assays employ a novel miRNA stem-loop primer during cDNA synthesis that produces a template for real-time PCR||TaqMan Advanced miRNAAssays employ a universal RT step for a streamlined workflow and a universal miR-Amp step to enable highly sensitive detection by real-time PCR.|
|RT chemistry||miRNA-specific RT||Universal RT|
|Throughput||Best for 1–10 targets||Best for >10 targets|
|Coverage||205 species available; coverage for miRBase v.2||All human, mouse, and rat miRNAs; coverage for miRBase v.22|
|Formats||Available in individual assays, pre-designed and custom 384-well, TaqMan array cards, and OpenArray™ formats||Available in individual assays, pre-designed 96-well TaqMan Array Plates, pre-designed and custom 384-well TaqMan Array Cards, and OpenArray formats.|
Ordering information - TaqMan Advanced miRNA Cards and Plates
Coverage of the most relevant microRNA for human species, aligned with Sanger miRBase v22, with over 740 assays enabled across the sets of TaqMan Advanced miRNA Array Cards or Plates. Requires the TaqMan Advanced cDNA synthesis kit (A28007) and recommended TaqMan Fast Advanced Master Mix.
For Research Use Only. Not for use in diagnostic procedures.