Let your colors show with iBright Imaging Systems

iBright 1500 Series Imaging Systems offer up to five imaging modes to support your applications. Efficiently and easily capture data from protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, and more. Get the most from your investment by having the freedom to choose from multiple detection strategies to meet your experimental needs.

Image modeWhat kind of signal can be captured?
Protein gelColorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain)
Nucleic acid gelEthidium bromide and Invitrogen SYBR stains
Chemiluminescent blotChemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Fluorescent blotFluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates)
UniversalCustom mode to image samples containing multiple signals, such as chemiluminescence, fluorescence, colorimetric stains, and/or visible images; image display is similar to fluorescent blot mode and allows one to assign false color to any sample

With the five fluorescent channels of the iBright FL1500 Imaging System, one can image with many different fluorophore options. Up to 4-channel multiplexing is possible, utilizing fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. The table below shows available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.

Fluorescence channels on the iBright FL1500 Imaging System

Excitation channelFilter range (nm)Emission channelFilter range (nm)
EX1455–485EM1508-557
EX2515–545EM2568–617
EX3608–632EM3675–720
EX4610–660EM4710–730
EX5745–765EM5800–850

Examples of fluorophores that can be detected using the iBright FL1500 Imaging System

Alexa Fluor DyesExcitation channelEmission channel
Alexa Fluor 488, Alexa Fluor Plus 488455-485 nm (EX1)508-557 nm (EM1)
Alexa Fluor 546515-545 nm (EX2)568-617 nm (EM2)
Alexa Fluor 555, Alexa Fluor Plus 555515-545 nm (EX2)568-617 nm (EM2)
Alexa Fluor 568515-545 nm (EX2)568-617 nm (EM2)
Alexa Fluor 594608-632 nm (EX3)675-720 nm (EM3)
Alexa Fluor 633608-632 nm (EX3)675-720 nm (EM3)
Alexa Fluor 647, Alexa Fluor Plus 647608-632 nm (EX3)675-720 nm (EM3)
Alexa Fluor 660610-660 nm (EX4)675-720 nm (EM3)
Alexa Fluor 680, Alexa Fluor Plus 680610-660 nm (EX4)710-730 nm (EM4)
Alexa Fluor 750745-765 nm (EX5)800-850 nm (EM5)
Alexa Fluor 790745-765 nm (EX5)800-850 nm (EM5)
Alexa Fluor Plus 800745-765 nm (EX5)800-850 nm (EM5)
DyLight DyesExcitation channelEmission channel
DyLight 488455-485 nm (EX1)508-557 nm (EM1)
DyLight 549/550515-545 nm (EX2)568-617 nm (EM2)
DyLight 594608-632 nm (EX3)675-720 nm (EM3)
DyLight 633608-632 nm (EX3)675-720 nm (EM3)
DyLight 649/650608-632 nm (EX3)675-720 nm (EM3)
DyLight 680610-660 nm (EX4)710-730 nm (EM4)
DyLight 755745-765 nm (EX5)800-850 nm (EM5)
DyLight 800745-765 nm (EX5)800-850 nm (EM5)
Western Dots, QdotsExcitation channelEmission channel
Qdots 525455-485 nm (EX1)508-557 nm (EM1)
Qdots 565455-485 nm (EX1)568-617 nm (EM2)
Qdots 585455-485 nm (EX1)568-617 nm (EM2)
Qdots 605455-485 nm (EX1)568-617 nm (EM2)
Qdots 625455-485 nm (EX1)568-617 nm (EM2)
Qdots 655455-485 nm (EX1)675-720 nm (EM3)
Qdots 705455-485 nm (EX1)675-720 nm (EM3)
Qdots 800455-485 nm (EX1)810-850 nm (EM5)
WesternDots 585455-485 nm (EX1)568-617 nm (EM2)
WesternDots 625455-485 nm (EX1)568-617 nm (EM2)
WesternDots 655455-485 nm (EX1)675-720 nm (EM3)
WesternDots 800455-485 nm (EX1)800-850 nm (EM5)
Traditional Dyes and CyaninExcitation channelEmission channel
Fluorescein/FITC455-485 nm (EX1)508-557 nm (EM1)
Green Fluorescent Protein (GFP)455-485 nm (EX1)508-557 nm (EM1)
Oregon Green455-485 nm (EX1)508-557 nm (EM1)
Red Fluorescent Protein (RFP)515-545 nm (EX2)568-617 nm (EM2)
Rhodamine 123455-485 nm (EX1)508-557 nm (EM1)
Tetramethyl Rhodamine (TRITC)515-545 nm (EX2)568-617 nm (EM2)
Texas Red608-632 nm (EX3)675-720 nm (EM3)
Cyanin 2 (Cy2)455-485 nm (EX1)508-557 nm (EM1)
Cyanin 3 (Cy3)515-545 nm (EX2)568-617 nm (EM2)
Cyanin 5 (Cy5)608-632 nm (EX3)675-720 nm (EM3)
Cyanin 5.5 (Cy5.5)610-660 nm (EX4)710-750 nm (EM4)
Cyanin 7 (Cy7)745-765 nm (EX5)800-850 nm (EM5)
IRDye, StarBrightExcitation channelEmission channel
IRDye 650608-632 nm (EX3)675-720 nm (EM3)
IRDye 680610-660 nm (EX4)710-730 nm (EM4)
IRDye 700610-660 nm (EX4)710-730 nm (EM4)
IRDye 800745-765 nm (EX5)800-850 nm (EM5)
StarBright Blue 520455-485 nm (EX1)508-557 nm (EM1)
StarBright Blue 700455-485 nm (EX1)710-730 nm (EM4)

Examples of chemiluminescent reagents that can be detected using the iBright FL1500 and CL1500 Imaging Systems

 FL1500CL1500
SuperSignal West Pico Plus  
SuperSignal West Dura  
SuperSignal West Femto  
Pierce ECL Plus  
Pierce ECL   
Other AP and HRP chemiluminescent substrates  

Example protein gel stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

 FL1500CL1500
Coomassie stains  
Silver stains  
SYPRO Ruby  
SYPRO Orange  
SYPRO Tangerine  
SYPRO Red  
Coomassie Fluor Orange  
Deep purple stains  
Oriole fluorescent stain  
Flamingo fluorescent stain  

Examples of nucleic acid dyes and stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

 FL1500CL1500
Ethidium bromide  
Hoechst blue  
SYBR Green I  
SYBR Green II  
SYBR Safe  
SYBR Gold  

With the five fluorescent channels of the iBright FL1500 Imaging System, one can image with many different fluorophore options. Up to 4-channel multiplexing is possible, utilizing fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. The table below shows available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.

Fluorescence channels on the iBright FL1500 Imaging System

Excitation channelFilter range (nm)Emission channelFilter range (nm)
EX1455–485EM1508-557
EX2515–545EM2568–617
EX3608–632EM3675–720
EX4610–660EM4710–730
EX5745–765EM5800–850

Examples of fluorophores that can be detected using the iBright FL1500 Imaging System

Alexa Fluor DyesExcitation channelEmission channel
Alexa Fluor 488, Alexa Fluor Plus 488455-485 nm (EX1)508-557 nm (EM1)
Alexa Fluor 546515-545 nm (EX2)568-617 nm (EM2)
Alexa Fluor 555, Alexa Fluor Plus 555515-545 nm (EX2)568-617 nm (EM2)
Alexa Fluor 568515-545 nm (EX2)568-617 nm (EM2)
Alexa Fluor 594608-632 nm (EX3)675-720 nm (EM3)
Alexa Fluor 633608-632 nm (EX3)675-720 nm (EM3)
Alexa Fluor 647, Alexa Fluor Plus 647608-632 nm (EX3)675-720 nm (EM3)
Alexa Fluor 660610-660 nm (EX4)675-720 nm (EM3)
Alexa Fluor 680, Alexa Fluor Plus 680610-660 nm (EX4)710-730 nm (EM4)
Alexa Fluor 750745-765 nm (EX5)800-850 nm (EM5)
Alexa Fluor 790745-765 nm (EX5)800-850 nm (EM5)
Alexa Fluor Plus 800745-765 nm (EX5)800-850 nm (EM5)
DyLight DyesExcitation channelEmission channel
DyLight 488455-485 nm (EX1)508-557 nm (EM1)
DyLight 549/550515-545 nm (EX2)568-617 nm (EM2)
DyLight 594608-632 nm (EX3)675-720 nm (EM3)
DyLight 633608-632 nm (EX3)675-720 nm (EM3)
DyLight 649/650608-632 nm (EX3)675-720 nm (EM3)
DyLight 680610-660 nm (EX4)710-730 nm (EM4)
DyLight 755745-765 nm (EX5)800-850 nm (EM5)
DyLight 800745-765 nm (EX5)800-850 nm (EM5)
Western Dots, QdotsExcitation channelEmission channel
Qdots 525455-485 nm (EX1)508-557 nm (EM1)
Qdots 565455-485 nm (EX1)568-617 nm (EM2)
Qdots 585455-485 nm (EX1)568-617 nm (EM2)
Qdots 605455-485 nm (EX1)568-617 nm (EM2)
Qdots 625455-485 nm (EX1)568-617 nm (EM2)
Qdots 655455-485 nm (EX1)675-720 nm (EM3)
Qdots 705455-485 nm (EX1)675-720 nm (EM3)
Qdots 800455-485 nm (EX1)810-850 nm (EM5)
WesternDots 585455-485 nm (EX1)568-617 nm (EM2)
WesternDots 625455-485 nm (EX1)568-617 nm (EM2)
WesternDots 655455-485 nm (EX1)675-720 nm (EM3)
WesternDots 800455-485 nm (EX1)800-850 nm (EM5)
Traditional Dyes and CyaninExcitation channelEmission channel
Fluorescein/FITC455-485 nm (EX1)508-557 nm (EM1)
Green Fluorescent Protein (GFP)455-485 nm (EX1)508-557 nm (EM1)
Oregon Green455-485 nm (EX1)508-557 nm (EM1)
Red Fluorescent Protein (RFP)515-545 nm (EX2)568-617 nm (EM2)
Rhodamine 123455-485 nm (EX1)508-557 nm (EM1)
Tetramethyl Rhodamine (TRITC)515-545 nm (EX2)568-617 nm (EM2)
Texas Red608-632 nm (EX3)675-720 nm (EM3)
Cyanin 2 (Cy2)455-485 nm (EX1)508-557 nm (EM1)
Cyanin 3 (Cy3)515-545 nm (EX2)568-617 nm (EM2)
Cyanin 5 (Cy5)608-632 nm (EX3)675-720 nm (EM3)
Cyanin 5.5 (Cy5.5)610-660 nm (EX4)710-750 nm (EM4)
Cyanin 7 (Cy7)745-765 nm (EX5)800-850 nm (EM5)
IRDye, StarBrightExcitation channelEmission channel
IRDye 650608-632 nm (EX3)675-720 nm (EM3)
IRDye 680610-660 nm (EX4)710-730 nm (EM4)
IRDye 700610-660 nm (EX4)710-730 nm (EM4)
IRDye 800745-765 nm (EX5)800-850 nm (EM5)
StarBright Blue 520455-485 nm (EX1)508-557 nm (EM1)
StarBright Blue 700455-485 nm (EX1)710-730 nm (EM4)

Examples of chemiluminescent reagents that can be detected using the iBright FL1500 and CL1500 Imaging Systems

 FL1500CL1500
SuperSignal West Pico Plus  
SuperSignal West Dura  
SuperSignal West Femto  
Pierce ECL Plus  
Pierce ECL   
Other AP and HRP chemiluminescent substrates  

Example protein gel stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

 FL1500CL1500
Coomassie stains  
Silver stains  
SYPRO Ruby  
SYPRO Orange  
SYPRO Tangerine  
SYPRO Red  
Coomassie Fluor Orange  
Deep purple stains  
Oriole fluorescent stain  
Flamingo fluorescent stain  

Examples of nucleic acid dyes and stains that can be detected using the iBright FL1500 and CL1500 Imaging Systems

 FL1500CL1500
Ethidium bromide  
Hoechst blue  
SYBR Green I  
SYBR Green II  
SYBR Safe  
SYBR Gold  
Visistat reference component