Cell Lysis and Fractionation

Our tissue lysis, cell lysis, and cell fractionation products are optimized for sample type. The lysates or fractions collected are compatible with a wide range of downstream applications. Features include:

  • High protein yields from cells or tissues
  • Gentle formulations that preserve protein activity for downstream assays
  • Directly compatible with protein assays, immunoprecipitation, immunoassays, western blotting, EMSA, and enzyme assays
  • Validated using multiple tissue types and cell lines
  • Eliminates the need for mechanical cell disruption

See western blotting resources and products ›

Featured cell lysis and fractionation categories

High-purity, low-peroxide, surfactant solutions and detergent solids for use in cell lysis reagent formulation, protein solubilization procedures, and as wash buffers for ELISA and other protein research methods.

Rapid, high-purity isolations from crude fractions or fractions of common densities—can be used to isolate a number of different organelles and organelle-specific proteins in small volumes, and can replace time-consuming and labor intensive density gradient centrifugations.

Gentle whole cell lysis buffers for high-yield protein extraction and solubilization from E. coli and other bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, and flowering plants.

Reagent-based kits optimized for stepwise separation, enrichment, and extraction of proteins from different cell fractions; isolate cytoplasmic, membrane, nuclear, chromatin-bound, and cytoskeletal proteins for downstream analysis.

Optimized reagent kits for subcellular fractionation of proteins and isolation of organelles such as nuclei, mitochondria, peroxisomes, and lysosomes by Dounce-homogenization and density-gradient separation.

Ready-to-use, inhibitor cocktail solutions and tablets, as well as individual purified formulations of protease inhibitors and phosphatase inhibitors to protect proteins from degradation during cell lysis and extraction.

Related categories

Mini- and microcentrifuges to accelerate your protein preparation protocols with RCFs up to 21,000 x g in compact, safe, easy-to-use instruments.

Designed for solid performance and consistent results, our benchtop centrifuges deliver efficient sample processing for your protein purification & isolation applications.

Featured product data

Nucleases and Enzymes