Saving Researchers from Tedious Manual Cell Counting for 10 Years

More accurate than manual cell counting

The Countess II instruments reduce variability and improve accuracy with the following key features:

• Autofocusing: Analyzing more than 30 focal planes, then selecting the plane with the best focal quality, for every count
• Auto-lighting: Optimizing lighting to maximize phase contrast objective for brightfield imaging
• Larger counting area: Detecting 3.5 times larger area than a single hemocytometer square used in manual cell counting estimations

Count cells in as little as 10 seconds

An individual counting five slides per day (two samples per slide) can save ~10 hours per month by switching to an automated counter with a reusable slide. The time savings increases to ~15 hours per month when using disposable slides.

Clumpy cells are not a problem

The advanced image processing algorithms of the Countess II Automated Cell Counters can clearly identify cell boundaries even within complex clumps that exceed 20 cells, resulting in more accurate cell counts.

Gating cells in real time

Cells can be gated in real time or ‘on-the-fly’ based on cell size, brightness, and circularity to fine-tune what cell attributes are included or excluded for specific applications.

Reusable slide is available

The mounting costs of disposable slides and tips have deterred many labs from giving up tedious manual cell counting. In response to this, the Countess II FL instrument was designed to work with a reusable glass slide that helps reduce the long-term consumable costs of automated counting significantly. Convenient disposable slides are also available.

More than just a brightfield channel

The Countess II FL instrument has two optional fluorescence channels in addition to one brightfield system. The fluorescence colors collected are determined by the insertion of individual light cubes. With 20 light cubes to choose from, the Countess II FL instrument is not limited to detecting only GFP- and RFP-expressing cells.

Cell viability assessment is more accurate

In addition to using the most commonly used method—trypan blue staining—for the brightfield channel, the Countess FL II instrument allows you to use up to two customizable fluorescence colors. This enables more accurate cell counts and viability assessment when dealing with complex samples containing significant amounts of platelets, RBCs, or debris.

Built-in dilution calculator makes your life easier

Easily determine the amount of cell sample and buffer needed for your experiment with the built-in dilution calculator. The cell count results are automatically used in the calculation; simply input the desired concentration and the volume needed.

Apoptosis and viability, in one count

The Countess II FL Automated Cell Counter equipped with GFP and Invitrogen Cy5 EVOS Light Cubes allows a quick and simple method to obtain apoptosis data together with cell viability data.

Transfection efficiency and viability, in one count

The Countess II FL Automated Cell Counter loaded with your choice of EVOS Light Cubes allows a quick and simple method to easily obtain transfection or transduction efficiency data together with cell viability data.