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The Applied Biosystems real-time PCR (qPCR) offering of integrated solutions is always evolving to keep up with today’s demands and tomorrow’s opportunities, including:

Great advancements have been made using Applied Biosystems real-time PCR instruments, beginning in the mid-1990s when the foundations of qPCR were laid. Over the decades, we’ve been designing and manufacturing qPCR instruments with ever-better features and innovations, which continue to accelerate scientific discoveries.

Learn more about our QuantStudio instruments below or request a quote now.

Fixed block real-time PCR systems

Simply accessible for new usersSimple to use for all levels of experienceEasy controls for optimized experiments



Interchangeable block real-time PCR systems

High flexibility and intuitive designSmart and connectedHigh versatility and intuitive designSmarter productivityMaximum throughput, flexibility, and scalability





Digital PCR system

Absolutely attainable chip-based digital PCR

Applied Biosystems TaqMan Assay technology is the gold standard in performance, quality, and content. We offer an end-to-end solution so you can spend your time generating results, rather than designing and optimizing assays. Our offering includes more than 20M predesigned assays, for more than 28 species, in single-tube, 96- or 384-well plate (including RFID-tagged plates for the QuantStudio Pro systems), 384-well microfluidic card, and OpenArray plate formats.


With so many choices to meet your unique needs, it’s hard to know where to start. That’s why we created an easy-to-use assay selection tool to help you.

Try our assay selection tool ›

Performance is guaranteed.

We want you to be confident in your results so we guarantee that all of our predesigned TaqMan Assays will enable you to obtain data you can trust, or we’ll replace them at no cost or give you a credit for the assay purchase price. Learn more about our TaqMan Guarantee.

Optimized real-time PCR master mixes, reagents, and kits to power your experiments

Recommended qPCR master mixes by application



Start with..

Applied Biosystems TaqMan*


TaqMan Fast Advanced Master Mix
for 2-step
TaqMan Fast Virus Master Mix  
for 1-step virus detection and gene expression

Applied Biosystems TaqPath**
(Clinical-grade TaqMan)


TaqPath qPCR Master Mix
for 2-step
TaqPath 1-step RT-qPCR Master Mix
for 1-step
TaqPath 1-step Multiplex Master Mix
for 1-step & multiplex


TaqPath ProAmp Master Mix
for human or animal samples only (for plant samples, please visit our Agrigenomics section)

Applied Biosystems SYBR


PowerTrack SYBR Green Master Mix

* For Research Use Only. Not for use in diagnostic procedures. ** General purpose reagent. For laboratory use. (Can also be used for research.)

Request a sample for non-clinical applications ›
Request a sample for clinical applications ›

Select from our full selection of optimized Applied Biosystems real-time PCR reagents to find the one that’s right for your desired application, detection chemistry, format, and running mode.

Try our real-time PCR master mix selection tool 

Successful real-time PCR experiments require a variety of products that are used before, during and after you use our QuantStudio Real-Time PCR systems.


For plastic consumables that have been validated for use with Applied Biosystems real-time PCR instruments and thermal cyclers, visit the On-line plastics selection tool. Easily find the plastics you need for all leading PCR and qPCR instruments.

Reverse transcriptases

Invitrogen SuperScript IV VILO Master Mix is a first-strand cDNA synthesis reaction mix for two-step RT-qPCR. The master mix format elevates the trusted VILO technology (Variable Input, Linear Output) to the next level by combining further optimized buffer conditions with highly processive and thermostable SuperScript IV Reverse Transcriptase (RT). This master mix offers exceptional performance features while maintaining superior linearity across the broadest range of input RNA.

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, PowerUp SYBR Green PCR Master Mix, and other PCR enzymes.


Our custom DNA oligos are made to your specifications, with rigorous quality control and quick turnaround. They can be used in a variety of applications, including PCR, cloning, sequencing, and gene detection.


See how well our products perform for free. Take the qPCR Challenge for a free t-shirt.* (Restrictions apply.)

**Get a free master mix sample ›

You may be surprised at how much your old Real-Time PCR instrument is worth. Find out today ›

Bundle and save on NGS and qPCR for SARS-CoV-2 research ›


Timeline poster

Get a free copy of our Real-Time PCR timeline poster that highlights innovations since the inception of this technology.***

Request your copy now ›

* No purchase necessary. This promotion is available only to life science professionals 21 years or older in the US (excluding Puerto Rico), and Canada, who are able to submit a completed survey. Healthcare Professionals may not participate in this promotion. Completed surveys must be received by Life Technologies no later than December 31, 2021, or until gift supplies are depleted, whichever comes first. The estimated value of the T-shirt is $9.60 USD. By submitting a survey, you warrant that you are not prohibited by employment, contract, or law from accepting a gift from Life Technologies. One gift per person. Please allow 8–10 weeks for delivery of the gift after submission of this form. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation.

** No purchase necessary. Available to life sciences professionals 21 years and older in the US (excluding Puerto Rico) and Canada. Offer will apply to sample requests submitted by December 31, 2021, or until promotional supplies are depleted. Sample size: 1 mL. Limit one sample per laboratory. Offer void where prohibited by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. View the privacy policy.