PG2152-PJT6527-COL112333-ET3-Gibco-Protein-Expression-Basics-Global-F

Gibco Protein Expression Basics is an online hub that brings together educational content and tools to enhance your research. Here you can access our protein expression selection guide, handbook, educational videos, and technical resources. Start here if you are new to protein expression or need to refresh your knowledge.

Gibco protein expression webinars

Watch on-demand webinars with leading industry experts and see the latest advances in protein expression.

COVID-19 Serology Assays and Cell Therapy Strategies

Presented by Fatima Amanat (Department of Microbiology, Icahn School of Medicine at Mount Sinai), Dean Lee (Director of the Cellular Therapy and Cancer Immunology Program and Physician at Nationwide Children's Hospital), and Lior Raviv (Vice President Development & Operations, Pluristem Therapeutics Inc.)

Harnessing the Power of Protein Expression Systems in Vaccine Development

Presented by Roland Leathers, PhD, Senior Manager, Strategic Alliances Europe, India & Australasia, Stem Cell Systems & Drug Discovery Solutions, Thermo Fisher Scientific

ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Presented by Ken Thompson, PhD

Expression, purification, and characterization of difficult to express membrane proteins in the Expi293 and ExpiCHO expression systems
Presented by Anass Jawhari, PhD, Chief Scientific Officer, CALIXAR

Enabling next-generation targeted siRNA therapies with ExpiCHO
Presented by Ian Huggins, PhD, Post-Doctoral Fellow, UC San Diego

Factor V expression in ExpiCHO cells
Presented by Laurent Mosnier, PhD, Associate Professor, The Scripps Research Institute

Expi293: new tools for structural biology and difficult to express proteins
Presented by Jon Zmuda, PhD, Director Cell Biology R&D, Thermo Fisher Scientific

Gibco ExpressionWorld 2016: Development of a mammalian recombinant protein production suite

Gibco ExpressionWorld 2016: Streamlining the drug development process with the ExpiCHO system

Protein Expression Publication Hub

Protein expression systems enable high-yield, consistent protein production for a diverse array of downstream applications. Protein solubility, functionality, purification speed, and yield are crucial factors to consider when choosing an expression system. Thermo Fisher Scientific drives innovation in the protein expression space, accelerating the development of new platforms that offer integrated solutions to accommodate your research needs.

This publication hub offers an in-depth look into how scientists like you advance their research with Expi protein expression systems. Our systems are optimized to harness the power of transient expression with the goal of delivering high-yield, consistent proteins for biotechnology, gene therapy, immunotherapy, immunology, structural, functional, vaccine, and infectious disease research.

Search the publication hub by filtering based on application, host type or expression system, or by entering a search term below.

  1. Applications:

  1. Host type:

  2. Expression system:

TitleJournalYearApplicationExpression System
Site-Specific Bioconjugation Using SMARTag® Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody–Drug ConjugatesMethods in Molecular Biology2019BiotechnologyExpiCHO
Optimizing high throughput antibody purification by using continuous chromatography mediaProtein Expression and Purification 2019BiotechnologyExpi293 and ExpiCHO
Simply Mixing Poly Protein G with Detection Antibodies Enhances the Detection Limit and Sensitivity of ImmunoassaysAnalytical Chemistry2019BiotechnologyExpi293
Site Selective Antibody-Oligonucleotide Conjugation via Microbial TransglutaminaseMolecules2019BiotechnologyExpiCHO
Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatographyNature Scientific Reports2018BiotechnologyExpi293
Transient CHO expression platform for robust antibody production and its enhanced N‐glycan sialylation on therapeutic glycoproteinsBiotechnology Progress2018BiotechnologyExpi293 and ExpiCHO
Scalable method utilizing low pH for DNA removal in the harvest of recombinant adeno-associated virus vectorsJournal of Chromatography B2019Gene Therapy Expi293
Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform Using the Herpes Simplex Virus SystemHuman Gene Therapy Methods2017Gene Therapy Expi293
Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector ProductionMolecular Therapy Methods and Clinical Development2020Gene Therapy ExpiSf
Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct VectorsMolecular Therapy Methods and Clinical Development2020Gene Therapy Expi293
Gene delivery of a modified antibody to Aβ reduces progression of murine Alzheimer’s diseasePLoS One2019ImmunotherapyExpi293
Vectored delivery of anti-SIV envelope targeting mAb via AAV8 protects rhesus macaques from repeated limiting dose intrarectal swarm SIVsmE660 challengePLoS Pathogens 2018ImmunotherapyExpi293
Establishment of P38Bf, a Core-Fucose-Deficient Mouse-Canine Chimeric Antibody Against Dog PodoplaninMonoclonal Antibodies in Immunodiagnosis and Immunotherapy2018ImmunotherapyExpiCHO
Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk regionEMBO Molecular Medicine 2020ImmunotherapyExpiCHO
Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52Frontiers in Immunology2019ImmunotherapyExpi293
Design and preclinical characterization of ALXN1210: a novel anti-C5 antibody with extended duration of actionPLOS One 2018ImmunotherapyExpi293
Antibody–Drug Conjugates Using Mouse–Canine Chimeric Anti-Dog Podoplanin Antibody Exerts Antitumor Activity in a Mouse Xenograft ModelMonoclonal Antibodies in Immunodiagnosis and Immunotherapy2020ImmunotherapyExpiCHO
Antibody-Based Targeting of Cell Surface GRP94 Specifically Inhibits Cetuximab-Resistant Colorectal Cancer GrowthBiomolecules2019ImmunotherapyExpi293
Blocking Monocytic Myeloid-Derived Suppressor Cell Function via Anti-DC-HIL/GPNMB Antibody Restores the In Vitro Integrity of T Cells from Cancer PatientsClinical Cancer Research2019ImmunotherapyExpiCHO
Dual targeting of RANKL and PD‐1 with a bispecific antibody improves anti‐tumor immunityClinical and Translational Immunology2019ImmunotherapyExpi293 and ExpiCHO
The Strong Propensity of Cadherin‐23 for Aggregation Inhibits Cell MigrationMolecular Oncology2019ImmunotherapyExpiCHO
Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncologyJournal of Allergy and Clinical Immunology2018ImmunotherapyExpi293
Differentiated agonistic antibody targeting CD137 eradicates large tumors without hepatotoxicityJournal of Clinical Investigation2020ImmunotherapyExpi293 and ExpiCHO
Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene Bcl-xL Combined with RapamycinBioprocess and Biosystems Engineering2018ImmunotherapyExpiCHO
Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologicsmAbs2020ImmunologyExpi293 and ExpiCHO
Engineering a high-affinity peptide binding site into the anti-CEA mAb M5AProtein Engineering Design & Selection2017ImmunologyExpiCHO
Human IgG subclass cross-species reactivity to mouse and cynomolgus monkey Fcγ receptorsImmunology Letters 2018ImmunologyExpi293
Early identification of unusually clustered mutations and root causes in therapeutic antibody developmentBiotechnology and Bioengineering 2018ImmunologyExpiCHO
Stabilization of the CD81 Large Extracellular Loop with De Novo Disulfide Bonds Improves Its Amenability for Peptide GraftingMDPI Pharmaceutics2018ImmunologyExpiCHO
Rapid charge variant analysis of monoclonal antibodies to support lead candidate biopharmaceutical developmentJournal of Chromatography B2018ImmunologyExpi293 and ExpiCHO
Lysosomal Protein Thermal Stability Does Not Correlate With Cellular Half-Life: Global Observations and a Case Study of Tripeptidyl-Peptidase 1Biochemical Journal 2020Protein Structure/FunctionExpiCHO
Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunctioneLife Science2019Protein Structure/FunctionExpiSf
Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized GlycoproteinInternational Journal of Analytical Chemistry 2018Protein Structure/FunctionExpiCHO
Mammalian plasma fetuin-B is a selective inhibitor of ovastacin and meprin metalloproteinasesNature Scientific Reports2019Protein Structure/FunctionExpiCHO
Tailored tetravalent antibodies potently and specifically activate Wnt/Frizzled pathways in cells, organoids and miceeLife Science2019Protein Structure/FunctionExpi293
IL-2 Shapes the Survival and Plasticity of IL-17–Producing γδ T CellsJournal of Immunology2017Protein Structure/FunctionExpi293
Identification of the gene that codes for the σ2 receptorPNAS2017Protein Structure/FunctionExpi293
Novel CH1:CL interfaces that enhance correct light chain pairing in heterodimeric bispecific antibodies Protein Engineering, Design and Selection2017Protein Structure/FunctionExpi293 and ExpiCHO
Mechanically interlocked functionalization of monoclonal antibodiesNature Communications2018Protein Structure/FunctionExpiCHO
Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regionsmAbs2018Protein Structure/FunctionExpiCHO
Pac1/LIS1 promotes an uninhibited conformation of dynein that coordinates its localization and activityNature Cell Biology2020Protein Structure/FunctionExpiSf
The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell InteractionMolecules2019Protein Structure/FunctionExpiCHO
Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibodyNature2020COVIDExpiCHO
Activation of latent transforming growth factor-β1, a conserved function for pregnancy-specific beta 1-glycoproteinsMolecular Human Reproduction2018Protein Structure/FunctionExpi293 and ExpiCHO
Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysisAMB Express2019Protein Structure/FunctionExpi293
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assaysProtein Expression and Purification 2020COVIDExpi293
Oligomeric state of the ZIKV E protein defines protective immune responsesNature Communications2019VaccineExpi293
In Vitro Assembly and Stabilization of Dengue and Zika Virus Envelope Protein Homo-DimersNature Scientific Reports2017VaccineExpi293
HIV-1 vaccine design through minimizing envelope metastabilityScience Advances 2018Vaccine ExpiCHO
Soluble Recombinant Hemagglutinin Protein of H1N1pdm09 Influenza Virus Elicits Cross-Protection Against a Lethal H5N1 Challenge in MiceFrontiers in Microbiology2019Vaccine Expi293
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotypePLOS Neglected Tropical Diseases 2018Vaccine Expi293
Synthetic DNA delivery by electroporation promotes robust in vivo sulfation of broadly neutralizing anti-HIV immunoadhesin eCD4-IgEBioMedicine 2018Vaccine Expi293
Neuraminidase Inhibition Contributes to Influenza A Virus Neutralization by Anti-Hemagglutinin Stem AntibodiesJournal of Experimental Medicine 2019Vaccine Expi293
Zika virus-like particle (VLP) based vaccinePLOS Neglected Tropical Diseases 2017Vaccine Expi293
Framework Mutations of the 10-1074 bnAb Increase Conformational Stability, Manufacturability, and Stability While Preserving Full Neutralization ActivityJournal of Pharmaceutical Sciences2019Vaccine ExpiCHO
Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig GeneCell Host & Microbe 2018Vaccine ExpiCHO
Broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomicseLife Science2019Vaccine Expi293 and ExpiCHO
Immunoglobulin fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitroAntiviral Research2020COVIDExpiCHO
Development of an inactivated vaccine candidate for SARS-CoV-2Science2020COVIDExpi293
Optimization of Surface Display of DENV2 E Protein on a Nanoparticle to Induce Virus Specific Neutralizing Antibody ResponsesBioconjugate Chemistry2018Vaccine Expi293
Proof of concept for rational design of hepatitis C virus E2 core nanoparticle vaccinesScience Advances 2020Vaccine ExpiCHO
Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational IncompatibilityCell Host & Microbe 2019Vaccine ExpiCHO
Crystal structures of human Fabs targeting the Bexsero meningococcal vaccine antigen NHBAActa Crystallographica Section F Structural Biology Communications2017Vaccine Expi293
SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test SetupCurrent Protocols in Microbiology2020COVIDExpi293
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformationScience2020COVIDExpi293
A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoVScience2020COVIDExpi293 and ExpiCHO
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigenPNAS2017COVIDExpi293
Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid AntibodiesCell2020COVIDExpiCHO
Cryo-EM structure of full-length HIV-1 Env bound with the Fab of antibody PG16Journal of Molecular Biology2020COVIDExpi293
Structure-based design of prefusion-stabilized SARS-CoV-2 spikesScience2020COVIDExpiCHO
Potent neutralizing antibodies directed to multiple epitopes on SARS-CoV-2 spikeNature 2020COVIDExpi293

Expi Expression Systems

Application notes

Protocol Calculator

Literature

Protocol guide

Scientific posters

Additional resources

Gibco protein expression webinars

Watch on-demand webinars with leading industry experts and see the latest advances in protein expression.

COVID-19 Serology Assays and Cell Therapy Strategies

Presented by Fatima Amanat (Department of Microbiology, Icahn School of Medicine at Mount Sinai), Dean Lee (Director of the Cellular Therapy and Cancer Immunology Program and Physician at Nationwide Children's Hospital), and Lior Raviv (Vice President Development & Operations, Pluristem Therapeutics Inc.)

Harnessing the Power of Protein Expression Systems in Vaccine Development

Presented by Roland Leathers, PhD, Senior Manager, Strategic Alliances Europe, India & Australasia, Stem Cell Systems & Drug Discovery Solutions, Thermo Fisher Scientific

ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Presented by Ken Thompson, PhD

Expression, purification, and characterization of difficult to express membrane proteins in the Expi293 and ExpiCHO expression systems
Presented by Anass Jawhari, PhD, Chief Scientific Officer, CALIXAR

Enabling next-generation targeted siRNA therapies with ExpiCHO
Presented by Ian Huggins, PhD, Post-Doctoral Fellow, UC San Diego

Factor V expression in ExpiCHO cells
Presented by Laurent Mosnier, PhD, Associate Professor, The Scripps Research Institute

Expi293: new tools for structural biology and difficult to express proteins
Presented by Jon Zmuda, PhD, Director Cell Biology R&D, Thermo Fisher Scientific

Gibco ExpressionWorld 2016: Development of a mammalian recombinant protein production suite

Gibco ExpressionWorld 2016: Streamlining the drug development process with the ExpiCHO system

Protein Expression Publication Hub

Protein expression systems enable high-yield, consistent protein production for a diverse array of downstream applications. Protein solubility, functionality, purification speed, and yield are crucial factors to consider when choosing an expression system. Thermo Fisher Scientific drives innovation in the protein expression space, accelerating the development of new platforms that offer integrated solutions to accommodate your research needs.

This publication hub offers an in-depth look into how scientists like you advance their research with Expi protein expression systems. Our systems are optimized to harness the power of transient expression with the goal of delivering high-yield, consistent proteins for biotechnology, gene therapy, immunotherapy, immunology, structural, functional, vaccine, and infectious disease research.

Search the publication hub by filtering based on application, host type or expression system, or by entering a search term below.

  1. Applications:

  1. Host type:

  2. Expression system:

TitleJournalYearApplicationExpression System
Site-Specific Bioconjugation Using SMARTag® Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody–Drug ConjugatesMethods in Molecular Biology2019BiotechnologyExpiCHO
Optimizing high throughput antibody purification by using continuous chromatography mediaProtein Expression and Purification 2019BiotechnologyExpi293 and ExpiCHO
Simply Mixing Poly Protein G with Detection Antibodies Enhances the Detection Limit and Sensitivity of ImmunoassaysAnalytical Chemistry2019BiotechnologyExpi293
Site Selective Antibody-Oligonucleotide Conjugation via Microbial TransglutaminaseMolecules2019BiotechnologyExpiCHO
Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatographyNature Scientific Reports2018BiotechnologyExpi293
Transient CHO expression platform for robust antibody production and its enhanced N‐glycan sialylation on therapeutic glycoproteinsBiotechnology Progress2018BiotechnologyExpi293 and ExpiCHO
Scalable method utilizing low pH for DNA removal in the harvest of recombinant adeno-associated virus vectorsJournal of Chromatography B2019Gene Therapy Expi293
Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform Using the Herpes Simplex Virus SystemHuman Gene Therapy Methods2017Gene Therapy Expi293
Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector ProductionMolecular Therapy Methods and Clinical Development2020Gene Therapy ExpiSf
Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct VectorsMolecular Therapy Methods and Clinical Development2020Gene Therapy Expi293
Gene delivery of a modified antibody to Aβ reduces progression of murine Alzheimer’s diseasePLoS One2019ImmunotherapyExpi293
Vectored delivery of anti-SIV envelope targeting mAb via AAV8 protects rhesus macaques from repeated limiting dose intrarectal swarm SIVsmE660 challengePLoS Pathogens 2018ImmunotherapyExpi293
Establishment of P38Bf, a Core-Fucose-Deficient Mouse-Canine Chimeric Antibody Against Dog PodoplaninMonoclonal Antibodies in Immunodiagnosis and Immunotherapy2018ImmunotherapyExpiCHO
Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk regionEMBO Molecular Medicine 2020ImmunotherapyExpiCHO
Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52Frontiers in Immunology2019ImmunotherapyExpi293
Design and preclinical characterization of ALXN1210: a novel anti-C5 antibody with extended duration of actionPLOS One 2018ImmunotherapyExpi293
Antibody–Drug Conjugates Using Mouse–Canine Chimeric Anti-Dog Podoplanin Antibody Exerts Antitumor Activity in a Mouse Xenograft ModelMonoclonal Antibodies in Immunodiagnosis and Immunotherapy2020ImmunotherapyExpiCHO
Antibody-Based Targeting of Cell Surface GRP94 Specifically Inhibits Cetuximab-Resistant Colorectal Cancer GrowthBiomolecules2019ImmunotherapyExpi293
Blocking Monocytic Myeloid-Derived Suppressor Cell Function via Anti-DC-HIL/GPNMB Antibody Restores the In Vitro Integrity of T Cells from Cancer PatientsClinical Cancer Research2019ImmunotherapyExpiCHO
Dual targeting of RANKL and PD‐1 with a bispecific antibody improves anti‐tumor immunityClinical and Translational Immunology2019ImmunotherapyExpi293 and ExpiCHO
The Strong Propensity of Cadherin‐23 for Aggregation Inhibits Cell MigrationMolecular Oncology2019ImmunotherapyExpiCHO
Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncologyJournal of Allergy and Clinical Immunology2018ImmunotherapyExpi293
Differentiated agonistic antibody targeting CD137 eradicates large tumors without hepatotoxicityJournal of Clinical Investigation2020ImmunotherapyExpi293 and ExpiCHO
Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene Bcl-xL Combined with RapamycinBioprocess and Biosystems Engineering2018ImmunotherapyExpiCHO
Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologicsmAbs2020ImmunologyExpi293 and ExpiCHO
Engineering a high-affinity peptide binding site into the anti-CEA mAb M5AProtein Engineering Design & Selection2017ImmunologyExpiCHO
Human IgG subclass cross-species reactivity to mouse and cynomolgus monkey Fcγ receptorsImmunology Letters 2018ImmunologyExpi293
Early identification of unusually clustered mutations and root causes in therapeutic antibody developmentBiotechnology and Bioengineering 2018ImmunologyExpiCHO
Stabilization of the CD81 Large Extracellular Loop with De Novo Disulfide Bonds Improves Its Amenability for Peptide GraftingMDPI Pharmaceutics2018ImmunologyExpiCHO
Rapid charge variant analysis of monoclonal antibodies to support lead candidate biopharmaceutical developmentJournal of Chromatography B2018ImmunologyExpi293 and ExpiCHO
Lysosomal Protein Thermal Stability Does Not Correlate With Cellular Half-Life: Global Observations and a Case Study of Tripeptidyl-Peptidase 1Biochemical Journal 2020Protein Structure/FunctionExpiCHO
Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunctioneLife Science2019Protein Structure/FunctionExpiSf
Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized GlycoproteinInternational Journal of Analytical Chemistry 2018Protein Structure/FunctionExpiCHO
Mammalian plasma fetuin-B is a selective inhibitor of ovastacin and meprin metalloproteinasesNature Scientific Reports2019Protein Structure/FunctionExpiCHO
Tailored tetravalent antibodies potently and specifically activate Wnt/Frizzled pathways in cells, organoids and miceeLife Science2019Protein Structure/FunctionExpi293
IL-2 Shapes the Survival and Plasticity of IL-17–Producing γδ T CellsJournal of Immunology2017Protein Structure/FunctionExpi293
Identification of the gene that codes for the σ2 receptorPNAS2017Protein Structure/FunctionExpi293
Novel CH1:CL interfaces that enhance correct light chain pairing in heterodimeric bispecific antibodies Protein Engineering, Design and Selection2017Protein Structure/FunctionExpi293 and ExpiCHO
Mechanically interlocked functionalization of monoclonal antibodiesNature Communications2018Protein Structure/FunctionExpiCHO
Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regionsmAbs2018Protein Structure/FunctionExpiCHO
Pac1/LIS1 promotes an uninhibited conformation of dynein that coordinates its localization and activityNature Cell Biology2020Protein Structure/FunctionExpiSf
The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell InteractionMolecules2019Protein Structure/FunctionExpiCHO
Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibodyNature2020COVIDExpiCHO
Activation of latent transforming growth factor-β1, a conserved function for pregnancy-specific beta 1-glycoproteinsMolecular Human Reproduction2018Protein Structure/FunctionExpi293 and ExpiCHO
Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysisAMB Express2019Protein Structure/FunctionExpi293
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assaysProtein Expression and Purification 2020COVIDExpi293
Oligomeric state of the ZIKV E protein defines protective immune responsesNature Communications2019VaccineExpi293
In Vitro Assembly and Stabilization of Dengue and Zika Virus Envelope Protein Homo-DimersNature Scientific Reports2017VaccineExpi293
HIV-1 vaccine design through minimizing envelope metastabilityScience Advances 2018Vaccine ExpiCHO
Soluble Recombinant Hemagglutinin Protein of H1N1pdm09 Influenza Virus Elicits Cross-Protection Against a Lethal H5N1 Challenge in MiceFrontiers in Microbiology2019Vaccine Expi293
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotypePLOS Neglected Tropical Diseases 2018Vaccine Expi293
Synthetic DNA delivery by electroporation promotes robust in vivo sulfation of broadly neutralizing anti-HIV immunoadhesin eCD4-IgEBioMedicine 2018Vaccine Expi293
Neuraminidase Inhibition Contributes to Influenza A Virus Neutralization by Anti-Hemagglutinin Stem AntibodiesJournal of Experimental Medicine 2019Vaccine Expi293
Zika virus-like particle (VLP) based vaccinePLOS Neglected Tropical Diseases 2017Vaccine Expi293
Framework Mutations of the 10-1074 bnAb Increase Conformational Stability, Manufacturability, and Stability While Preserving Full Neutralization ActivityJournal of Pharmaceutical Sciences2019Vaccine ExpiCHO
Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig GeneCell Host & Microbe 2018Vaccine ExpiCHO
Broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomicseLife Science2019Vaccine Expi293 and ExpiCHO
Immunoglobulin fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitroAntiviral Research2020COVIDExpiCHO
Development of an inactivated vaccine candidate for SARS-CoV-2Science2020COVIDExpi293
Optimization of Surface Display of DENV2 E Protein on a Nanoparticle to Induce Virus Specific Neutralizing Antibody ResponsesBioconjugate Chemistry2018Vaccine Expi293
Proof of concept for rational design of hepatitis C virus E2 core nanoparticle vaccinesScience Advances 2020Vaccine ExpiCHO
Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational IncompatibilityCell Host & Microbe 2019Vaccine ExpiCHO
Crystal structures of human Fabs targeting the Bexsero meningococcal vaccine antigen NHBAActa Crystallographica Section F Structural Biology Communications2017Vaccine Expi293
SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test SetupCurrent Protocols in Microbiology2020COVIDExpi293
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformationScience2020COVIDExpi293
A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoVScience2020COVIDExpi293 and ExpiCHO
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigenPNAS2017COVIDExpi293
Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid AntibodiesCell2020COVIDExpiCHO
Cryo-EM structure of full-length HIV-1 Env bound with the Fab of antibody PG16Journal of Molecular Biology2020COVIDExpi293
Structure-based design of prefusion-stabilized SARS-CoV-2 spikesScience2020COVIDExpiCHO
Potent neutralizing antibodies directed to multiple epitopes on SARS-CoV-2 spikeNature 2020COVIDExpi293

Expi Expression Systems

Application notes

Protocol Calculator

Literature

Protocol guide

Scientific posters

Additional resources

Gibco Protein Expression Basics videos